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Catalog Products » Antibodies » MonoRab™ Rabbit Monoclonal Antibody Product » MonoRab™ DYKDDDDK Tag Antibody [HRP], mAb, Rabbit

MonoRab™ DYKDDDDK Tag Antibody [HRP], mAb, Rabbit

MonoRab™ DYKDDDDK Tag Antibody [HRP], MAb, Rabbit

MonoRabᵀᴹ DYKDDDDK Tag Antibody [HRP], mAb, Rabbit (GenScript, A01869-40) binds with DYKDDDDK Tag.
Coating antigen: DYKDDDDK-tagged fusion protein, 1 µg/ml.
MonoRabᵀᴹ DYKDDDDK Tag Antibody [HRP], mAb, Rabbit (GenScript, A01869-40) dilution start from 1,000 ng/ml.
EC50= 20.21 ng/ml.

MonoRab™ DYKDDDDK Tag Antibody [HRP], MAb, Rabbit

Sensitivity and specificity of MonoRabᵀᴹ DYKDDDDK Tag Antibody [HRP], mAb, Rabbit (GenScript, A01869-40) to C-DYKDDDDK-tag Fusion Protein by Western blot.
Loading:
Lane 1 : C-terminal DYKDDDDK-tagged fusion protein (25 ng)
Lane 2 : C-terminal DYKDDDDK-tagged fusion protein (10 ng)
Lane 3 : C-terminal DYKDDDDK-tagged fusion protein (5 ng)
Lane 4 : C-terminal DYKDDDDK-tagged fusion protein (1 ng)
Primary Antibody:
MonoRabᵀᴹ DYKDDDDK Tag Antibody [HRP], mAb, Rabbit (GenScript, A01869-40) 1 μg/ml

MonoRab™ DYKDDDDK Tag Antibody [HRP], MAb, Rabbit

Sensitivity and specificity of MonoRabᵀᴹ DYKDDDDK Tag Antibody [HRP], mAb, Rabbit (GenScript, A01869-40) to N-DYKDDDDK-tag Fusion Protein by Western blot.
Loading:
Lane 1 : N-terminal DYKDDDDK-tagged fusion protein (25 ng)
Lane 2 : N-terminal DYKDDDDK-tagged fusion protein (10 ng)
Lane 3 : N-terminal DYKDDDDK-tagged fusion protein (5 ng)
Lane 4 : N-terminal DYKDDDDK-tagged fusion protein (1 ng)
Primary Antibody:
MonoRabᵀᴹ DYKDDDDK Tag Antibody [HRP], mAb, Rabbit (GenScript, A01869-40) 1 μg/ml

Specificity GenScript MonoRab™ DYKDDDDK Tag Antibody [HRP], mAb, Rabbit specifically recognizes DYKDDDDK tags placed at C-terminal, N-terminal and internal regions of DYKDDDDK fusion proteins.
Host Species Rabbit
Immunogen DYKDDDDK synthetic peptide coupled to KLH
Conjugate Horseradish peroxidase (HRP)

Working concentrations for specific applications should be determined by the investigator. The appropriate concentration may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.05-0.2 μg/ml
Western Blot 0.5-1 μg/ml 
Other applications User-optimized

Form Lyophilized
Storage Buffer lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.
Storage Instructions The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Purification Protein A affinity column
Isotype Rabbit IgG
Clonality Monoclonal
Clone ID 114F12C8
Note GenScript can customize this product per customer's request including product size, buffer components, etc.

  • MonoRab™ DYKDDDDK Tag Antibody [HRP], MAb, Rabbit
  • MonoRab™ DYKDDDDK Tag Antibody [HRP], MAb, Rabbit

    MonoRabᵀᴹ DYKDDDDK Tag Antibody [HRP], mAb, Rabbit (GenScript, A01869-40) binds with DYKDDDDK Tag.
    Coating antigen: DYKDDDDK-tagged fusion protein, 1 µg/ml.
    MonoRabᵀᴹ DYKDDDDK Tag Antibody [HRP], mAb, Rabbit (GenScript, A01869-40) dilution start from 1,000 ng/ml.
    EC50= 20.21 ng/ml.

  • MonoRab™ DYKDDDDK Tag Antibody [HRP], MAb, Rabbit
  • MonoRab™ DYKDDDDK Tag Antibody [HRP], MAb, Rabbit

    Sensitivity and specificity of MonoRabᵀᴹ DYKDDDDK Tag Antibody [HRP], mAb, Rabbit (GenScript, A01869-40) to C-DYKDDDDK-tag Fusion Protein by Western blot.
    Loading:
    Lane 1 : C-terminal DYKDDDDK-tagged fusion protein (25 ng)
    Lane 2 : C-terminal DYKDDDDK-tagged fusion protein (10 ng)
    Lane 3 : C-terminal DYKDDDDK-tagged fusion protein (5 ng)
    Lane 4 : C-terminal DYKDDDDK-tagged fusion protein (1 ng)
    Primary Antibody:
    MonoRabᵀᴹ DYKDDDDK Tag Antibody [HRP], mAb, Rabbit (GenScript, A01869-40) 1 μg/ml

  • MonoRab™ DYKDDDDK Tag Antibody [HRP], MAb, Rabbit
  • MonoRab™ DYKDDDDK Tag Antibody [HRP], MAb, Rabbit

    Sensitivity and specificity of MonoRabᵀᴹ DYKDDDDK Tag Antibody [HRP], mAb, Rabbit (GenScript, A01869-40) to N-DYKDDDDK-tag Fusion Protein by Western blot.
    Loading:
    Lane 1 : N-terminal DYKDDDDK-tagged fusion protein (25 ng)
    Lane 2 : N-terminal DYKDDDDK-tagged fusion protein (10 ng)
    Lane 3 : N-terminal DYKDDDDK-tagged fusion protein (5 ng)
    Lane 4 : N-terminal DYKDDDDK-tagged fusion protein (1 ng)
    Primary Antibody:
    MonoRabᵀᴹ DYKDDDDK Tag Antibody [HRP], mAb, Rabbit (GenScript, A01869-40) 1 μg/ml


Target Background The rabbit immune system generates antibody diversity and optimizes affinity. GenScript utilizes MonoRab™ technology to generate the high affinity and specificity monoclonal rabbit antibodies. MonoRab™ DYKDDDDK Tag Antibody [HRP], mAb, Rabbit specific to DYKDDDDK tags placed at C-terminal, N-terminal and internal regions of fusion proteins. The antibody can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify DYKDDDDK fusion proteins in bacteria and mammalian cells.
Synonyms Rabbit monoclonal to Flag

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


Cat. No. Product Name Quantity Price
A01870-40
MonoRab™ DYKDDDDK Tag Antibody [Biotin], mAb, Rabbit - 40 µg
$198.00
A01871-40
MonoRab™ DYKDDDDK Tag Antibody [FITC], mAb, Rabbit - 40 µg
$198.00
A01868-40
MonoRab™ DYKDDDDK Tag Antibody, mAb, Rabbit - 40 µg
$198.00
 


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Cat. No. A01869
Size Price
40 µg $198.00