|Description||CRISPR/Cas9 system is one powerful gene editing tool to specifically modify target genes. Cas9 nuclease is an RNA-guided endonuclease that can catalyze cleavage of double stranded DNA. The GenCrispr Cas9 Antibody is a rabbit polyclonal antibody with Streptococcus pyogene (S. pyogenes) Cas9 protein as the immunization antigen. This antibody is used to detect the expression level of CRISPR/Cas9 in target cells.|
|Antigen Species||Streptococcus pyogene|
|Antigen Synonyms||Cas9 protein|
|Immunogen||Recombinant Streptococcus pyogene (S. pyogenes) Cas9 protein.|
|Specificity||This antibody recognizes Streptococcus pyogene (S. pyogenes) Cas9 protein (UniProt Accession: Q99ZW2). Other species have not been not tested.|
|Storage||The GenCrispr Cas9 Antibody is liquid in PBS, pH 7.4, containing 50% glycerol. The recommended storage temperature is -20°C.|
|Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended as starting points for this product.
WB: 1:1000 - 1:5000.
Other applications: user-optimized.
High Sensitivity: GenCrispr Cas9 antibody can detect as low as 0.5 ng GenCrispr Cas9 nuclease.
GenCrispr Cas9 protein (GenScript, Cat.No. Z03386) was loaded on a gel as indicated and then detected by Western blot using GenCrispr Cas9 antibody (1:3000 dilution) and Goat Anti-Rabbit IgG (H&L) [HRP],pAb (GenScript, Cat No. A10169) (1: 10000 dilution).