ELISA binding of MonoRabᵀᴹ Anti-Adalimumab Antibody (4E12), mAb, Rabbit (GenScript, A01921-40) with Adalimumab. While the antibody does not recognize the human IgG Fc fragment (data not shown).
Coating antigen: Adalimumab, 1 µg/ml.
Anti-Adalimumab antibody (GenScript, A01921-40) dilution start from 1,250 ng/ml.
EC₅₀= 6.283 ng/ml.
MonoRabᵀᴹ Anti-Adalimumab Antibody (4E12), mAb, Rabbit (GenScript, A01921-40) blocks Adalimumab binding with TNF-α.
Coating antigen: Adalimumab, 0.5 µg/ml.
Anti-Adalimumab Antibody (4E12), mAb, Rabbit (GenScript, A01921-40) final concentration: 3.5 ng/ml.
TNF-α dilution start from 50 µg/ml.
IC₅₀= 175.4 ng/ml.
Standard curve of Adalimumab Sandwich ELISA. The Adalimumab Sandwich ELISA assay is developed by using MonoRabᵀᴹ Anti-Adalimumab Antibody (4E12), mAb, Rabbit (GenScript, A01921-40) and MonoRabᵀᴹ Anti-Adalimumab Antibody (134D5), mAb, Rabbit (GenScript, A01922-40) as the capture and detection antibodies, respectively. These two antibodies could be used to development Sandwich ELISA Kit.
In this ELISA assay, MonoRabᵀᴹ Anti-Adalimumab Antibody (134D5), mAb, Rabbit (GenScript, A01922-40) was labeled with Biotin. GenScript can provide customized conjugation services for this product per customer's request.
The sensitivity is <2 ng/ml and the detection range is 0-33 ng/ml.
MonoRab™ Anti-Adalimumab Antibody (4E12), mAb, Rabbit
A01921 | |
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$211.00 | |
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