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Catalog Products » GenCrispr Cas9 Genome Editing Products » GenCrispr Cas9 Antibodies » GenCRISPRᵀᴹ SaCas9 Antibody (11C12), mAb, Mouse

GenCRISPRᵀᴹ SaCas9 Antibody (11C12), mAb, Mouse

GenCRISPRᵀᴹ SaCas9 Antibody (11C12), MAb, Mouse

ELISA binding of GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40) with recombinant Staphylococcus aureus CRISPR/Cas9 protein.
Coating antigen: SaCas9, 1 µg/ml.
SaCas9 antibody dilution start from 1000 ng/ml,EC₅₀= 3.75 ng/ml.

GenCRISPRᵀᴹ SaCas9 Antibody (11C12), MAb, Mouse

Immunofluorescence staining of GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40) with SaCas9 in Hela cells.
HeLa cells transfected with PX458 (SaCas9(BB)-2A-GFP) (colored green) were fixed with 4% Poly-Formaldehyde (5min) and then blocked in 3% BSA 30min.
The cells were then incubated with GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40) at 0.5 μg/ml mouse IgG at 0.5 μg/ml at room temperature for 2h, followed by a further incubation at 37°C for 1h with Goat Anti-Mouse IgG Antibody (H&L) [ifluor 555], pAb (GenScript) (colored red) at 5 μg/ml.
DAPI was used to stain the cell nuclei (colored blue) at a concentration of 0.2 μg/ml for 2h at room temperature.

GenCRISPRᵀᴹ SaCas9 Antibody (11C12), MAb, Mouse

Standard curve of SaCas9 Sandwich ELISA. The SaCas9 Sandwich ELISA assay is developed by using GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40) and GenCRISPRᵀᴹ SaCas9 Antibody (26H10) (GenScript, A01952-40) as capture and detection antibody, respectively. These two antibodies recognize different epitopes.
In this ELISA assay, GenCRISPRᵀᴹ SaCas9 Antibody (26H10) (GenScript, A01952-40) was labeled with Biotin. GenScript can provide customized conjugation service for this product per customer's request.
The sensitivity is <1 ng/ml and the detection range is 0-30 ng/ml.

GenCRISPRᵀᴹ SaCas9 Antibody (11C12), MAb, Mouse

Western Blot of Hela transfected with PX458 (SaCas9(BB)-2A-GFP) or untransfected cell lysates with two independent antibodies: GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40) and GenCRISPRᵀᴹ SaCas9 Antibody (26H10) (GenScript, A01952-40). The correlated pattern indicates the high specificity of these two antibodies.
Predicted band size: 124 kDa
Predicted band size of recombinant protein: 47.5 kDa
Loading:
Lane 1: 50 μg Hela transfected with SaCas9(BB)-2A-GFP cell Lysate
Lane 2: 50 μg Untransfected Hela cell Lysate
Lane 3: 40 ng SaCas9 recombinant protein
Lane 4: 50 μg Hela transfected with SaCas9(BB)-2A-GFP cell Lysate
Lane 5: 50 μg Untransfected Hela cell Lysate
Lane 6: 40 ng SaCas9 recombinant protein
Primary Antibody:
Lane 1~3: GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40) 1 μg/ml
Lane 4~6: GenCRISPRᵀᴹ SaCas9 Antibody (26H10) (GenScript, A01952-40) 1 μg/ml
Secondary Antibody:
Goat anti-Mouse IgG (H&L) [IRDye⁸⁰⁰] (Licor,926-32211) 0.125 μg/ml

GenCRISPRᵀᴹ SaCas9 Antibody (11C12), MAb, Mouse

Western Blot of Hela transfected with PX458 (SaCas9(BB)-2A-GFP) or untransfected cell lysates with GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40). The different concentration of cell lysates indicates the high affinity and sensitivity of the antibody.
Predicted band size: 124 kDa
Predicted band size of recombinant protein: 47.5 kDa
Loading:
Lane 1: 50 μg Hela transfected with SaCas9(BB)-2A-GFP cell Lysate
Lane 2: 25 μg Hela transfected with SaCas9(BB)-2A-GFP cell Lysate
Lane 3: 10 μg Hela transfected with SaCas9(BB)-2A-GFP cell Lysate
Lane 4: 50 μg Untransfected Hela cell Lysate
Lane 5: 40 ng SaCas9 recombinant protein
Primary Antibody:
GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40) 1 μg/ml
Secondary Antibody:
Goat anti-Mouse IgG (H&L) [IRDye⁸⁰⁰] (Licor,926-32211) 0.125 μg/ml

GenCRISPRᵀᴹ SaCas9 Antibody (11C12), MAb, Mouse

Western Blot analysis of Hela transfected with various plasmids with GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40). The different Hela transfected with various plasmids indicate the minimum cross reaction of the antibody.
Predicted band size: 124 kDa
Loading:
Lane 1: 50μg Hela cell lysate transfected with StCas9(BB)-2A-GFP (G3ECR1, Streptococcus thermophiles)
Lane 2: 50μg Hela cell lysate transfected with pSpCas9(BB)-2A-GFP (PX458, Q99ZW2, Streptococcus pyogenes serotype M1)
Lane 3: 50μg Hela cell lysate transfected with SaCas9(BB)-2A-GFP (J7RUA5, Staphylococcus aureus)
Lane 4: 50μg Hela cell lysate transfected with AsCpf1(BB)-2A-GFP (U2UMQ6, Acidaminococcus sp. (strain BV3L6))
Lane 5: 50μg Hela cell lysate transfected with FnCpf1(BB)-2A-GFP (A0Q7Q2, Francisella tularensis subsp. novicida (strain U112))
Lane 6: 50μg Hela cell lysate transfected with LbCpf1(BB)-2A-GFP (A0A182DWE3, Lachnospiraceae bacterium ND2006)
Lane 7: 50μg Hela cell lysate (Non-transfected)
Primary Antibody:
GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40) 1 μg/ml
Secondary antibody:
Goat anti-Mouse IgG (H&L) [IRDye⁸⁰⁰] (Licor,926-32211) 0.125 μg/ml

GenCRISPRᵀᴹ SaCas9 Antibody (11C12), MAb, Mouse

Immunoprecipitation of SaCas9 from Hela cell transfected with PX458 (SaCas9(BB)-2A-GFP) by using GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40).
Predicted band size: 124 kDa
Loading:
Lane 1: Hela cell lysate transfected with PX458 (SaCas9(BB)-2A-GFP) 50μg (Input)
Lane 2: Protein A MagBeads (20μl) + Purified antibody of A01951-40 (10μg) + Hela cell lysate transfected with PX458 (SaCas9(BB)-2A-GFP) (200μg)
Lane 3: Protein A MagBeads (20μl) + Purified antibody of A01951-40 (10μg) + Hela cell lysate (200μg)
Lane 4: Protein A MagBeads (20μl) + Mouse IgG (10μg) + Hela cell lysate transfected with PX458 (SaCas9(BB)-2A-GFP) (200μg)
Lane 5: Protein A MagBeads (20μl) + Mouse IgG (10μg) + Hela Cell lysate (200μg)
Lane 6: Hela Cell lysate (50μg)
Primary Antibody:
GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40) 1 μg/ml
Secondary antibody:
Goat anti-Mouse IgG (H&L) [IRDye⁸⁰⁰] (Licor,926-32211) 0.125 μg/ml

Description Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) protein 9 system provides a robust and multiplexable genome editing tool, enabling researchers to precisely manipulate specific genomic elements, and facilitating the elucidation of target gene function in biology and diseases. CRISPR/Cas9 genome editing allows for double-stranded DNA breaks at specific sequences to efficiently disrupt, excise, mutate, insert, or replace genes. The best characterized CRISPR-associated nucleases are the Cas9 proteins from Streptococcus pyogenes and Staphylococcus aureus. It is important that the precision of transfection and the level of Cas9 expression should be controlled strictly during the editing processes by using specific anti-CRISPR/Cas9 antibodies.
Host Species Mouse
Conjugation Unconjugated
Immunogen Recombinant Staphylococcus aureus CRISPR/Cas9
Purification Protein A affinity column

Subclass IgG2b,K
Clone ID 11C12
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, contains 0.02% sodium azide. (Might be eligible for customized bulk orders.)
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity The product is specific for Staphylococcus aureus CRISPR/Cas9. This antibody binds with recombinant Staphylococcus aureus CRISPR/Cas9 protein in ELISA and Endogenous overexpressed Staphylococcus aureus CRISPR/Cas9 in immunofluorescence, western blot, immunoprecipitation.
Storage The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid freeze/thaw cycles.
Note GenScript can customize this product per customer's request including product size, buffer components, etc.

Working concentrations for specific applications should be determined by the investigators. The appropriate concentration may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA detection: 0.005-1 µg/ml
Immunofluorescence: 0.2-1 µg/ml
Western blot: 1 µg/ml
Other applications: user-optimized

  • GenCRISPRᵀᴹ SaCas9 Antibody (11C12), MAb, Mouse
  • GenCRISPRᵀᴹ SaCas9 Antibody (11C12), MAb, Mouse

    ELISA binding of GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40) with recombinant Staphylococcus aureus CRISPR/Cas9 protein.
    Coating antigen: SaCas9, 1 µg/ml.
    SaCas9 antibody dilution start from 1000 ng/ml,EC₅₀= 3.75 ng/ml.

  • GenCRISPRᵀᴹ SaCas9 Antibody (11C12), MAb, Mouse
  • GenCRISPRᵀᴹ SaCas9 Antibody (11C12), MAb, Mouse

    Immunofluorescence staining of GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40) with SaCas9 in Hela cells.
    HeLa cells transfected with PX458 (SaCas9(BB)-2A-GFP) (colored green) were fixed with 4% Poly-Formaldehyde (5min) and then blocked in 3% BSA 30min.
    The cells were then incubated with GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40) at 0.5 μg/ml mouse IgG at 0.5 μg/ml at room temperature for 2h, followed by a further incubation at 37°C for 1h with Goat Anti-Mouse IgG Antibody (H&L) [ifluor 555], pAb (GenScript) (colored red) at 5 μg/ml.
    DAPI was used to stain the cell nuclei (colored blue) at a concentration of 0.2 μg/ml for 2h at room temperature.

  • GenCRISPRᵀᴹ SaCas9 Antibody (11C12), MAb, Mouse
  • GenCRISPRᵀᴹ SaCas9 Antibody (11C12), MAb, Mouse

    Standard curve of SaCas9 Sandwich ELISA. The SaCas9 Sandwich ELISA assay is developed by using GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40) and GenCRISPRᵀᴹ SaCas9 Antibody (26H10) (GenScript, A01952-40) as capture and detection antibody, respectively. These two antibodies recognize different epitopes.
    In this ELISA assay, GenCRISPRᵀᴹ SaCas9 Antibody (26H10) (GenScript, A01952-40) was labeled with Biotin. GenScript can provide customized conjugation service for this product per customer's request.
    The sensitivity is <1 ng/ml and the detection range is 0-30 ng/ml.

  • GenCRISPRᵀᴹ SaCas9 Antibody (11C12), MAb, Mouse
  • GenCRISPRᵀᴹ SaCas9 Antibody (11C12), MAb, Mouse

    Western Blot of Hela transfected with PX458 (SaCas9(BB)-2A-GFP) or untransfected cell lysates with two independent antibodies: GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40) and GenCRISPRᵀᴹ SaCas9 Antibody (26H10) (GenScript, A01952-40). The correlated pattern indicates the high specificity of these two antibodies.
    Predicted band size: 124 kDa
    Predicted band size of recombinant protein: 47.5 kDa
    Loading:
    Lane 1: 50 μg Hela transfected with SaCas9(BB)-2A-GFP cell Lysate
    Lane 2: 50 μg Untransfected Hela cell Lysate
    Lane 3: 40 ng SaCas9 recombinant protein
    Lane 4: 50 μg Hela transfected with SaCas9(BB)-2A-GFP cell Lysate
    Lane 5: 50 μg Untransfected Hela cell Lysate
    Lane 6: 40 ng SaCas9 recombinant protein
    Primary Antibody:
    Lane 1~3: GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40) 1 μg/ml
    Lane 4~6: GenCRISPRᵀᴹ SaCas9 Antibody (26H10) (GenScript, A01952-40) 1 μg/ml
    Secondary Antibody:
    Goat anti-Mouse IgG (H&L) [IRDye⁸⁰⁰] (Licor,926-32211) 0.125 μg/ml

  • GenCRISPRᵀᴹ SaCas9 Antibody (11C12), MAb, Mouse
  • GenCRISPRᵀᴹ SaCas9 Antibody (11C12), MAb, Mouse

    Western Blot of Hela transfected with PX458 (SaCas9(BB)-2A-GFP) or untransfected cell lysates with GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40). The different concentration of cell lysates indicates the high affinity and sensitivity of the antibody.
    Predicted band size: 124 kDa
    Predicted band size of recombinant protein: 47.5 kDa
    Loading:
    Lane 1: 50 μg Hela transfected with SaCas9(BB)-2A-GFP cell Lysate
    Lane 2: 25 μg Hela transfected with SaCas9(BB)-2A-GFP cell Lysate
    Lane 3: 10 μg Hela transfected with SaCas9(BB)-2A-GFP cell Lysate
    Lane 4: 50 μg Untransfected Hela cell Lysate
    Lane 5: 40 ng SaCas9 recombinant protein
    Primary Antibody:
    GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40) 1 μg/ml
    Secondary Antibody:
    Goat anti-Mouse IgG (H&L) [IRDye⁸⁰⁰] (Licor,926-32211) 0.125 μg/ml

  • GenCRISPRᵀᴹ SaCas9 Antibody (11C12), MAb, Mouse
  • GenCRISPRᵀᴹ SaCas9 Antibody (11C12), MAb, Mouse

    Western Blot analysis of Hela transfected with various plasmids with GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40). The different Hela transfected with various plasmids indicate the minimum cross reaction of the antibody.
    Predicted band size: 124 kDa
    Loading:
    Lane 1: 50μg Hela cell lysate transfected with StCas9(BB)-2A-GFP (G3ECR1, Streptococcus thermophiles)
    Lane 2: 50μg Hela cell lysate transfected with pSpCas9(BB)-2A-GFP (PX458, Q99ZW2, Streptococcus pyogenes serotype M1)
    Lane 3: 50μg Hela cell lysate transfected with SaCas9(BB)-2A-GFP (J7RUA5, Staphylococcus aureus)
    Lane 4: 50μg Hela cell lysate transfected with AsCpf1(BB)-2A-GFP (U2UMQ6, Acidaminococcus sp. (strain BV3L6))
    Lane 5: 50μg Hela cell lysate transfected with FnCpf1(BB)-2A-GFP (A0Q7Q2, Francisella tularensis subsp. novicida (strain U112))
    Lane 6: 50μg Hela cell lysate transfected with LbCpf1(BB)-2A-GFP (A0A182DWE3, Lachnospiraceae bacterium ND2006)
    Lane 7: 50μg Hela cell lysate (Non-transfected)
    Primary Antibody:
    GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40) 1 μg/ml
    Secondary antibody:
    Goat anti-Mouse IgG (H&L) [IRDye⁸⁰⁰] (Licor,926-32211) 0.125 μg/ml

  • GenCRISPRᵀᴹ SaCas9 Antibody (11C12), MAb, Mouse
  • GenCRISPRᵀᴹ SaCas9 Antibody (11C12), MAb, Mouse

    Immunoprecipitation of SaCas9 from Hela cell transfected with PX458 (SaCas9(BB)-2A-GFP) by using GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40).
    Predicted band size: 124 kDa
    Loading:
    Lane 1: Hela cell lysate transfected with PX458 (SaCas9(BB)-2A-GFP) 50μg (Input)
    Lane 2: Protein A MagBeads (20μl) + Purified antibody of A01951-40 (10μg) + Hela cell lysate transfected with PX458 (SaCas9(BB)-2A-GFP) (200μg)
    Lane 3: Protein A MagBeads (20μl) + Purified antibody of A01951-40 (10μg) + Hela cell lysate (200μg)
    Lane 4: Protein A MagBeads (20μl) + Mouse IgG (10μg) + Hela cell lysate transfected with PX458 (SaCas9(BB)-2A-GFP) (200μg)
    Lane 5: Protein A MagBeads (20μl) + Mouse IgG (10μg) + Hela Cell lysate (200μg)
    Lane 6: Hela Cell lysate (50μg)
    Primary Antibody:
    GenCRISPRᵀᴹ SaCas9 Antibody (11C12) (GenScript, A01951-40) 1 μg/ml
    Secondary antibody:
    Goat anti-Mouse IgG (H&L) [IRDye⁸⁰⁰] (Licor,926-32211) 0.125 μg/ml




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Cat. No. A01951
Size
Price
40 µg $99.00
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