Catalog Products » Antibodies » MonoRab™ Rabbit Monoclonal Antibody Product » MonoRab™ FITC Antibody (25H7), mAb, Rabbit

MonoRab™ FITC Antibody (25H7), mAb, Rabbit

*This product has been discontinued!*
GenScript MonoRab™ Fluorescein isothiocyanate Antibody (25H7), mAb, Rabbit is developed using Fluorescein isothiocyanate-KLH as immunogen which is specific to Fluorescein isothiocyanate. Fluorescein isothiocyanate antibody reacts with both free and conjugated fluorescein isothiocyanate.
A01953
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Overview
Specificity GenScript MonoRab™ Fluorescein isothiocyanate Antibody (25H7), mAb, Rabbit is developed using Fluorescein isothiocyanate-KLH as immunogen which is specific to Fluorescein isothiocyanate. Fluorescein isothiocyanate antibody reacts with both free and conjugated fluorescein isothiocyanate.
Host Species Rabbit
Immunogen Fluorescein isothiocyanate coupled to KLH
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentration may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.01-0.1 μg/ml

Properties
Form Lyophilized
Storage Buffer lyophilized with PBS, pH 7.4, contains 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.
Storage Instructions The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid freeze/thaw cycles.
Purification Protein A affinity column
Isotype Rabbit IgG
Clonality Monoclonal
Clone ID 25H7
Note GenScript can customize this product per customer's request including product size, buffer components, etc.

Background
Target Background Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins.
Synonyms Rabbit monoclonal to Fluorescein isothiocyanate/Fluorescein

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


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