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MonoRabᵀᴹ Anti-Atezolizumab Antibody (43H11), mAb, Rabbit

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ELISA binding of MonoRabᵀᴹ Anti-Atezolizumab Antibody (43H11) (GenScript, A01985-40) with Atezolizumab. While the antibody does not recognize the human IgG Fc fragment (data not shown).
Coating antigen: Atezolizumab, 1 µg/ml.
MonoRabᵀᴹ Anti-Atezolizumab Antibody (43H11) (GenScript, A01985-40) dilutions start from 1,000 ng/ml.
EC₅₀= 3.749 ng/ml.

Standard curve of Atezolizumab Sandwich ELISA. The Atezolizumab Sandwich ELISA assay is developed by using MonoRabᵀᴹ Anti-Atezolizumab Antibody (55D8), mAb, Rabbit (GenScript, A01984-40) and MonoRabᵀᴹ Anti-Atezolizumab Antibody (43H11) (Genscript, A01985-40) as the capture and detection antibodies, respectively.
In this ELISA assay, MonoRabᵀᴹ Anti-Atezolizumab Antibody (43H11) (GenScript, A01985-40) was labeled with Biotin. GenScript can provide customized conjugation services for this product per the customer's request.The sensitivity of detecting Atezolizumab is up to 156 pg/ml.

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Overview

Synonyms	Rabbit monoclonal to Tecentriq
Description	Atezolizumab is a fully humanized monoclonal antibody that is approved by the U.S. Food and Drug Administration for the treatment of metastatic non-small cell lung cancer (NSCLC). It binds to the protein programmed cell death-ligand 1 (PD-L1) on several tumors. Atezolizumab has ability to block the binding of PD-L1 with PD-1 and CD80 receptors (B7-1Rs), which has a positive effect on improving an anti-tumor response. GenScript MonoRabᵀᴹ Anti-Atezolizumab Antibody (43H11), mAb, Rabbit is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with Atezolizumab.
Host Species	Rabbit
Conjugation	Unconjugated
Immunogen	Atezolizumab
Purification	Protein A affinity column

Properties

Subclass	IgG
Clone ID	43H11
Concentration	0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution	Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity	The antibody is recommended as detection antibody in a pharmacokinetic (PK) bridging assay with captrue antibody GenScript, A01984-40, MonoRab Anti-Atezolizumab Antibody (55D8), mAb, Rabbit.
Storage	The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Note	GenScript can customize this product per customer's request including product size, buffer components, etc.

Applications

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA detection: 0.01-0.1 µg/ml
Other applications: user-optimized