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Catalog Products » Antibodies » MonoRab™ Rabbit Monoclonal Antibody Product » MonoRab™ Anti-Nivolumab Antibody (33H4), mAb, Rabbit

MonoRab™ Anti-Nivolumab Antibody (33H4), mAb, Rabbit

MonoRab™ Anti-Nivolumab Antibody (33H4), MAb, Rabbit

ELISA binding of MonoRabᵀᴹ Anti-Nivolumab Antibody (33H4) (GenScript, A01987-40) with Nivolumab. While the antibody does not recognize the human IgG Fc fragment (data not shown).
Coating antigen: Nivolumab, 1 µg/ml.
MonoRabᵀᴹ Anti-Nivolumab Antibody (33H4) (GenScript, A01987-40) dilutions start from 1,000 ng/ml.
EC₅₀= 22.48 ng/ml.

MonoRab™ Anti-Nivolumab Antibody (33H4), MAb, Rabbit

Standard curve of Nivolumab Sandwich ELISA. The Nivolumab Sandwich ELISA assay is developed by using MonoRabᵀᴹ Anti-Nivolumab Antibody (33H4), mAb, Rabbit (GenScript, A01987-40) and MonoRabᵀᴹ Anti-Nivolumab Antibody (40F11) (Genscript, A01988-40) as the capture and detection antibodies, respectively.
In this ELISA assay, MonoRabᵀᴹ Anti-Nivolumab Antibody (40F11) (Genscript, A01988-40) was labeled with Biotin. GenScript can provide customized conjugation services for this product per the customer's request.
The sensitivity of detecting Nivolumab is up to 78 pg/ml.

MonoRab™ Anti-Nivolumab Antibody (33H4), MAb, Rabbit

MonoRabᵀᴹ Anti-Nivolumab Antibody (33H4), mAb, Rabbit (GenScript, A01987-40) blocks Nivolumab binding with Human PD-1 recombinant protein (Z03370, PD-1 Fc Chimera, Human).
Coating antigen: Nivolumab, 1 μg/ml.
PD-1-Fc-biotin final concentration: 10 ng/ml.
MonoRabᵀᴹ Anti-Nivolumab Antibody (33H4), mAb, Rabbit (GenScript, A01987-40) dilutions start from 10 μg/ml.IC₅₀= 0.48 μg/ml

Specificity The antibody is recommended as a capture antibody in a pharmacokinetic (PK) bridging assay with detection antibody GenScript, A01988-40, MonoRab Anti-Nivolumab Antibody (40F11), mAb, Rabbit.
Host Species Rabbit
Immunogen Nivolumab
Conjugate Unconjugated

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA detection 0.01-0.1 µg/ml
Other applications User-optimized

Form Lyophilized
Storage Buffer Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Storage Instructions The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Purification Protein A affinity column
Isotype Rabbit IgG
Clonality Monoclonal
Clone ID 33H4
Note GenScript can customize this product per customer's request including product size, buffer components, etc.

  • MonoRab™ Anti-Nivolumab Antibody (33H4), MAb, Rabbit
  • MonoRab™ Anti-Nivolumab Antibody (33H4), MAb, Rabbit

    ELISA binding of MonoRabᵀᴹ Anti-Nivolumab Antibody (33H4) (GenScript, A01987-40) with Nivolumab. While the antibody does not recognize the human IgG Fc fragment (data not shown).
    Coating antigen: Nivolumab, 1 µg/ml.
    MonoRabᵀᴹ Anti-Nivolumab Antibody (33H4) (GenScript, A01987-40) dilutions start from 1,000 ng/ml.
    EC₅₀= 22.48 ng/ml.

  • MonoRab™ Anti-Nivolumab Antibody (33H4), MAb, Rabbit
  • MonoRab™ Anti-Nivolumab Antibody (33H4), MAb, Rabbit

    Standard curve of Nivolumab Sandwich ELISA. The Nivolumab Sandwich ELISA assay is developed by using MonoRabᵀᴹ Anti-Nivolumab Antibody (33H4), mAb, Rabbit (GenScript, A01987-40) and MonoRabᵀᴹ Anti-Nivolumab Antibody (40F11) (Genscript, A01988-40) as the capture and detection antibodies, respectively.
    In this ELISA assay, MonoRabᵀᴹ Anti-Nivolumab Antibody (40F11) (Genscript, A01988-40) was labeled with Biotin. GenScript can provide customized conjugation services for this product per the customer's request.
    The sensitivity of detecting Nivolumab is up to 78 pg/ml.

  • MonoRab™ Anti-Nivolumab Antibody (33H4), MAb, Rabbit
  • MonoRab™ Anti-Nivolumab Antibody (33H4), MAb, Rabbit

    MonoRabᵀᴹ Anti-Nivolumab Antibody (33H4), mAb, Rabbit (GenScript, A01987-40) blocks Nivolumab binding with Human PD-1 recombinant protein (Z03370, PD-1 Fc Chimera, Human).
    Coating antigen: Nivolumab, 1 μg/ml.
    PD-1-Fc-biotin final concentration: 10 ng/ml.
    MonoRabᵀᴹ Anti-Nivolumab Antibody (33H4), mAb, Rabbit (GenScript, A01987-40) dilutions start from 10 μg/ml.IC₅₀= 0.48 μg/ml


Target Background Nivolumab is a fully human monoclonal IgG4 antibody that is approved by the U.S. Food and Drug Administration for the trea™ent of bladder cancer. It binds to the programmed cell death protein 1 (PD-1) on the surface of activated T cells. Nivolumab functions as PD-1 inhibitor for targeted immunotherapy.GenScript MonoRab™ Anti-Nivolumab Antibody (33H4), mAb, Rabbit is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with Nivolumab.
Synonyms Rabbit monoclonal to Opdivo

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.




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Cat. No. A01987
Size Price
40 µg $198.00