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MonoRab^TM Anti-Pembrolizumab Antibody (94B9), mAb, Rabbit

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MonoRab<sup>TM</sup> Anti-Pembrolizumab Antibody (94B9), MAb, Rabbit

ELISA binding of MonoRab^TM Anti-Pembrolizumab Antibody (94B9) (GenScript, A02008-40) with Pembrolizumab. While the antibody does not recognize the human IgG Fc fragment (data not shown).
Coating antigen: Pembrolizumab, 1 µg/ml.
MonoRab^TM Anti-Pembrolizumab Antibody (94B9) (GenScript, A02008-40) dilutions start from 1,000 ng/ml.
EC₅₀= 2.234 ng/ml.

Standard curve of Pembrolizumab Sandwich ELISA. The Pembrolizumab Sandwich ELISA assay is developed by using MonoRab^TM Anti-Pembrolizumab Antibody (94B9), mAb, Rabbit (GenScript, A02008-40) and MonoRab^TM Anti-Pembrolizumab Antibody (47E5) (Genscript, A02007-40) as the capture and detection antibodies, respectively.
In this ELISA assay, MonoRab^TM Anti-Pembrolizumab Antibody (47E5) (GenScript, A02007-40) was labeled with Biotin. GenScript can provide customized conjugation services for this product per the customer's request.
The sensitivity of detecting Pembrolizumab is up to 78 pg/ml.

MonoRab^TM Anti-Pembrolizumab Antibody (94B9), mAb, Rabbit (GenScript, A02008-40) blocks Pembrolizumab binding with Human PD-1 protein (Z03370, PD 1-Fc Chimera, Human).
Coating antigen: PD-1 protein, 0.5 µg/ml
Pembrolizumab-biotin final concentration: 20 ng/ml
MonoRab^TM Anti-Pembrolizumab Antibody (94B9), mAb, Rabbit (GenScript, A02008-40) dilutions start from 30 μg/ml
IC₅₀= 580 ng/ml

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Overview

Description	Pembrolizumab (Keytruda) is a humanized monoclonal antibody that is approved by the U.S. Food and Drug Administration for the treatment of patients with unresectable or metastatic melanoma. Pembrolizumab binds to the human cell surface receptor PD-1 (Programmed Cell Death Protein 1) and blocks its interaction with PD-L1, resulting in the activation of T-cell-mediated immune responses against tumor cells. GenScript MonoRab^TM Anti-Pembrolizumab Antibody (94B9), mAb, Rabbit is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with Pembrolizumab.
Host Species	Rabbit
Conjugation	Unconjugated
Immunogen	Pembrolizumab
Purification	Protein A affinity column

Properties

Subclass	IgG
Clone ID	94B9
Concentration	0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution	Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity	The antibody is recommended as a capture antibody in a pharmacokinetic (PK) bridging assay with detection antibody GenScript, A02007-40, MonoRab™ Anti-Pembrolizumab Antibody (47E5), mAb, Rabbit.
Storage	The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Note	GenScript can customize this product per customer's request including product size, buffer components, etc.

Applications

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA detection: 0.01-0.1 µg/ml
ELISA blocking: 10-50 µg/ml
Other applications: user-optimized