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MonoRabTM Anti-Pembrolizumab Antibody (94B9), mAb, Rabbit
MonoRab™ Anti-Pembrolizumab Antibody (94B9), mAb, Rabbit
ELISA binding of MonoRabTM Anti-Pembrolizumab Antibody (94B9) (GenScript, A02008-40) with Pembrolizumab. While the antibody does not recognize the human IgG Fc fragment (data not shown).
Coating antigen: Pembrolizumab, 1 µg/ml.
MonoRabTM Anti-Pembrolizumab Antibody (94B9) (GenScript, A02008-40) dilutions start from 1,000 ng/ml.
EC50= 2.234 ng/ml.
Standard curve of Pembrolizumab Sandwich ELISA. The Pembrolizumab Sandwich ELISA assay is developed by using MonoRabTM Anti-Pembrolizumab Antibody (94B9), mAb, Rabbit (GenScript, A02008-40) and MonoRabTM Anti-Pembrolizumab Antibody (47E5) (Genscript, A02007-40) as the capture and detection antibodies, respectively.
In this ELISA assay, MonoRabTM Anti-Pembrolizumab Antibody (47E5) (GenScript, A02007-40) was labeled with Biotin. GenScript can provide customized conjugation services for this product per the customer's request.
The sensitivity of detecting Pembrolizumab is up to 78 pg/ml.
MonoRabTM Anti-Pembrolizumab Antibody (94B9), mAb, Rabbit (GenScript, A02008-40) blocks Pembrolizumab binding with Human PD-1 protein (Z03370, PD 1-Fc Chimera, Human).
Coating antigen: PD-1 protein, 0.5 µg/ml
Pembrolizumab-biotin final concentration: 20 ng/ml
MonoRabTM Anti-Pembrolizumab Antibody (94B9), mAb, Rabbit (GenScript, A02008-40) dilutions start from 30 μg/ml
IC50= 580 ng/ml
| Synonyms | Rabbit monoclonal to Keytruda |
| Description | Pembrolizumab (Keytruda) is a humanized monoclonal antibody that is approved by the U.S. Food and Drug Administration for the treatment of patients with unresectable or metastatic melanoma. Pembrolizumab binds to the human cell surface receptor PD-1 (Programmed Cell Death Protein 1) and blocks its interaction with PD-L1, resulting in the activation of T-cell-mediated immune responses against tumor cells. GenScript MonoRabTM Anti-Pembrolizumab Antibody (94B9), mAb, Rabbit is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with Pembrolizumab. |
| Host Species | Rabbit |
| Conjugation | Unconjugated |
| Immunogen | Pembrolizumab |
| Purification | Protein A affinity column |
| Subclass | IgG |
| Clone ID | 94B9 |
| Concentration | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Reconstitution | Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml. |
| Specificity | The antibody is recommended as a capture antibody in a pharmacokinetic (PK) bridging assay with detection antibody GenScript, A02007-40, MonoRab™ Anti-Pembrolizumab Antibody (47E5), mAb, Rabbit. |
| Storage | The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles. |
| Note | GenScript can customize this product per customer's request including product size, buffer components, etc. |
| Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA detection: 0.01-0.1 µg/ml
ELISA blocking: 10-50 µg/ml
Other applications: user-optimized |
ELISA binding of MonoRabTM Anti-Pembrolizumab Antibody (94B9) (GenScript, A02008-40) with Pembrolizumab. While the antibody does not recognize the human IgG Fc fragment (data not shown).
Coating antigen: Pembrolizumab, 1 µg/ml.
MonoRabTM Anti-Pembrolizumab Antibody (94B9) (GenScript, A02008-40) dilutions start from 1,000 ng/ml.
EC50= 2.234 ng/ml.
Standard curve of Pembrolizumab Sandwich ELISA. The Pembrolizumab Sandwich ELISA assay is developed by using MonoRabTM Anti-Pembrolizumab Antibody (94B9), mAb, Rabbit (GenScript, A02008-40) and MonoRabTM Anti-Pembrolizumab Antibody (47E5) (Genscript, A02007-40) as the capture and detection antibodies, respectively.
In this ELISA assay, MonoRabTM Anti-Pembrolizumab Antibody (47E5) (GenScript, A02007-40) was labeled with Biotin. GenScript can provide customized conjugation services for this product per the customer's request.
The sensitivity of detecting Pembrolizumab is up to 78 pg/ml.
MonoRabTM Anti-Pembrolizumab Antibody (94B9), mAb, Rabbit (GenScript, A02008-40) blocks Pembrolizumab binding with Human PD-1 protein (Z03370, PD 1-Fc Chimera, Human).
Coating antigen: PD-1 protein, 0.5 µg/ml
Pembrolizumab-biotin final concentration: 20 ng/ml
MonoRabTM Anti-Pembrolizumab Antibody (94B9), mAb, Rabbit (GenScript, A02008-40) dilutions start from 30 μg/ml
IC50= 580 ng/ml
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
A01845-40 |
Anti-Pembrolizumab Antibody(10E12C6)[Biotin], mAb, Mouse - 40 ug |
$139.00 |
|
A01844-40 |
Anti-Pembrolizumab Antibody(1D6E10), mAb, Mouse - 40 ug |
$139.00 |
For more documents, please visit "Technical Support".
| Cat. No. A02008 | ||
|---|---|---|
| Size | Price | |
| 40 ug | $198.00 | |
