Western Blot analysis of MonoRabTM SARS-CoV-2 Neutralizing Antibody (4G6), mAb, Rabbit (GenScript, A02053) with SARS-CoV-2 RBD.
Lane 1: Negative cell supernatant of RBD ,10 μl
Lane 2: Positive cell supernatant of RBD , 10 μl
Lane 3: SARS-CoV-2 RBD Protein , 20 ng
Primary Antibody:
MonoRabTM SARS-CoV-2 Neutralizing Antibody (4G6), mAb, Rabbit , 1 μg/ml
Secondary Antibody:
Anti-Rabbit IgG (H&L) Goat Antibody Peroxidase Conjugated
ELISA binding of MonoRabTM SARS-CoV-2 Neutralizing Antibody (4G6), mAb, Rabbit (GenScript, A02053) with recombinant SARS-CoV-2 Spike Protein S1 RBD(GenScript, Z03483) and SARS-CoV RBD.
Synonyms | 2019-nCoV Neutralizing Antibody, mAb, Rabbit |
Description | SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2), also known as 2019-nCoV, is a positive-sense single-stranded RNA virus. It caused coronavirus disease 2019 (COVID-19). SARS-CoV-2 contains glycosylated spike (S) protein, which is composed of S1 subunit and S2 subunit. The S1 contains a receptor-binding domain (RBD) that can bind to ACE2 receptor on target cells. |
Host Species | Rabbit |
Conjugation | Unconjugated |
Immunogen | Recombinant SARS-CoV-2 Spike protein fragment |
Purification | Protein A |
Clone | 4G6 (monoclonal antibody) |
Subclass | Recombinant IgG |
Concentration | 1 mg/ml, 0.2 μm filtered solution in PBS, pH 7.4 |
Specificity | The product is specific for SARS-CoV-2 Spike Protein S1 subunit and its RBD domain. |
Storage | Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed. Avoid repeated freezing and thawing cycles. |
Note | GenScript can customize this product per customer's request including product size, buffer components, etc. |
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA detection: 0.01-0.1 µg/ml Neutralization (Neu) assay: 0.2-1 µg/ml FACS: 10 µg/ml Western blot: 1 µg/ml Other applications: user-optimized |
Western Blot analysis of MonoRabTM SARS-CoV-2 Neutralizing Antibody (4G6), mAb, Rabbit (GenScript, A02053) with SARS-CoV-2 RBD.
Lane 1: Negative cell supernatant of RBD ,10 μl
Lane 2: Positive cell supernatant of RBD , 10 μl
Lane 3: SARS-CoV-2 RBD Protein , 20 ng
Primary Antibody:
MonoRabTM SARS-CoV-2 Neutralizing Antibody (4G6), mAb, Rabbit , 1 μg/ml
Secondary Antibody:
Anti-Rabbit IgG (H&L) Goat Antibody Peroxidase Conjugated
ELISA binding of MonoRabTM SARS-CoV-2 Neutralizing Antibody (4G6), mAb, Rabbit (GenScript, A02053) with recombinant SARS-CoV-2 Spike Protein S1 RBD(GenScript, Z03483) and SARS-CoV RBD.
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