ELISA binding of SARS-CoV-2 ADE-Causing Antibody (S9HC) (GenScript, A02133) with recombinant SARS-CoV-2 Spike protein (ECD, His & Flag Tag) (GenScript, Z03481).
Coating antigen: SARS-CoV-2 Spike protein (ECD), 1 µg/ml.
SARS-CoV-2 ADE-Causing Antibody (S9HC) (GenScript, A02133) dilution start from 1 µg/ml.
Binding assay of SARS-COV-2 (Delta) to ACE2 mediated by antibodies
First, recombinant SARS-CoV-2 Delta spike protein (S1, His Tag) (GenScript, Z03612) was coated on the plate. Then, ADE-Causing antibody (GenScript, A02133), neutralizing antibody (GenScript, A02087) or non-neutralizing antibody (GenScript, A02038) were added to the wells after gradient dilution. Last, HRP conjugated ACE2 protein was added to the well to form the signals.
The result indicated that SARS-CoV-2 ADE-Causing Antibody (S9HC) (A02133) could enhance SARS-CoV-2 Delta spike protein binding with ACE2 compared with A02087 and A02038 which proved the ADE effect.
SARS-CoV-2 ADE-Causing Antibody (S9HC)
A02133 | |
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$375.00 | |
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