Catalog Products » SARS-CoV-2 Nucleocapsid Antibody (N338), mAb, mouse
SARS-CoV-2 Nucleocapsid Antibody (N338), MAb, Mouse

Standard curve of SARS-CoV-2 Nucleocapsid Protein LFA Assay.

The LFA assay is developed by using SARS-CoV-2 Nucleocapsid Antibody (N338), mAb, mouse(GenScript, A02135) and MonoRab™ SARS-CoV-2 Nucleocapsid Antibody (N34), mAb, Rabbit(GenScript, A02136) as the capture and detection antibodies, respectively.

The combination shows good dose response of fluorescence intensity and concentrations of SARS-CoV-2 Nucleocapsid protein in LFA assay.

Nucleocapsid protein concentration was nicely correlated with fluorescence intensity.

SARS-CoV-2 Nucleocapsid Antibody (N338), MAb, Mouse

Western Blot of wild-type and delta variant Nucleocapsid protein cell lysates with SARS-CoV-2 Nucleocapsid Antibody (N338), mAb, mouse (GenScript, A02135).

M: Protein Marker (GenScript, M00521)
Lane 1: 3 μg Wild-type N protein cell lysate
Lane 2: 3 μg Delta N protein cell lysate

Primary Antibody:
SARS-CoV-2 Nucleocapsid Antibody (N338), mAb, mouse (GenScript, A02135) 1 μg/ml

Secondary Antibody:
Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in rabbit (Sigma, A9044)

SARS-CoV-2 Nucleocapsid Antibody (N338), MAb, Mouse

BLI (Biolayer interferometry) binding affinity measurements of SARS-CoV-2 Nucleocapsid Antibody (N338), mAb, mouse(GenScript, A02135) to SARS-CoV-2 Nucleocapsid protein of wild type and delta variant.

SARS-CoV-2 Nucleocapsid Antibody (N338) captured on Protein A sensors surface can bind wild-type or delta variant with dissociation constant (KD) of 3.62E-09M and 1.58E-09M.

SARS-CoV-2 Nucleocapsid Antibody (N338), MAb, Mouse

BLI (Biolayer interferometry) binding affinity measurements of SARS-CoV-2 Nucleocapsid Antibody (N338), mAb, mouse (GenScript, A02135) to nucleocapsid protein of SARS-CoV-2 omicron variant.

SARS-CoV-2 Nucleocapsid Antibody (N338) captured on Protein A sensors surface can bind omicron variant with dissociation constant (KD) of 5.56E-09M.

SARS-CoV-2 Nucleocapsid Antibody (N338), mAb, mouse

GenScript SARS-CoV-2 Nucleocapsid Antibody (N338), mAb, mouse is specific for SARS-CoV-2 Nucleocapsid protein of wild type, delta variant, and omicron variant.
A02135
$279.00

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Overview
Specificity GenScript SARS-CoV-2 Nucleocapsid Antibody (N338), mAb, mouse is specific for SARS-CoV-2 Nucleocapsid protein of wild type, delta variant, and omicron variant.
Host Species Mouse
Immunogen Recombinant SARS-CoV-2 Nucleocapsid protein
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 1:1,000 - 1:5,000
Chemiluminescent immunoassay (CLIA) 1:1,000 - 1:5,000
Western Blot 1:100 - 1:1,000
Lateral flow assay (LFA) A02135(C) - A02136 (D); A02135(C) - A02138(D)

Properties
Form Liquid
Storage Buffer PBS, pH 7.2, 0.02% sodium azide
Concentration 1 mg/ml
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed. Avoid repeated freezing and thawing cycles.
Purification Affinity chromatography
Isotype Mouse IgG
Clonality Monoclonal
Clone ID N338
Note GenScript can customize this product per customer's request including product size, buffer components, etc.

Examples
  • SARS-CoV-2 Nucleocapsid Antibody (N338), MAb, Mouse
  • SARS-CoV-2 Nucleocapsid Antibody (N338), MAb, Mouse

    Standard curve of SARS-CoV-2 Nucleocapsid Protein LFA Assay.

    The LFA assay is developed by using SARS-CoV-2 Nucleocapsid Antibody (N338), mAb, mouse(GenScript, A02135) and MonoRab™ SARS-CoV-2 Nucleocapsid Antibody (N34), mAb, Rabbit(GenScript, A02136) as the capture and detection antibodies, respectively.

    The combination shows good dose response of fluorescence intensity and concentrations of SARS-CoV-2 Nucleocapsid protein in LFA assay.

    Nucleocapsid protein concentration was nicely correlated with fluorescence intensity.

  • SARS-CoV-2 Nucleocapsid Antibody (N338), MAb, Mouse
  • SARS-CoV-2 Nucleocapsid Antibody (N338), MAb, Mouse

    Western Blot of wild-type and delta variant Nucleocapsid protein cell lysates with SARS-CoV-2 Nucleocapsid Antibody (N338), mAb, mouse (GenScript, A02135).

    M: Protein Marker (GenScript, M00521)
    Lane 1: 3 μg Wild-type N protein cell lysate
    Lane 2: 3 μg Delta N protein cell lysate

    Primary Antibody:
    SARS-CoV-2 Nucleocapsid Antibody (N338), mAb, mouse (GenScript, A02135) 1 μg/ml

    Secondary Antibody:
    Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in rabbit (Sigma, A9044)

  • SARS-CoV-2 Nucleocapsid Antibody (N338), MAb, Mouse
  • SARS-CoV-2 Nucleocapsid Antibody (N338), MAb, Mouse

    BLI (Biolayer interferometry) binding affinity measurements of SARS-CoV-2 Nucleocapsid Antibody (N338), mAb, mouse(GenScript, A02135) to SARS-CoV-2 Nucleocapsid protein of wild type and delta variant.

    SARS-CoV-2 Nucleocapsid Antibody (N338) captured on Protein A sensors surface can bind wild-type or delta variant with dissociation constant (KD) of 3.62E-09M and 1.58E-09M.

  • SARS-CoV-2 Nucleocapsid Antibody (N338), MAb, Mouse
  • SARS-CoV-2 Nucleocapsid Antibody (N338), MAb, Mouse

    BLI (Biolayer interferometry) binding affinity measurements of SARS-CoV-2 Nucleocapsid Antibody (N338), mAb, mouse (GenScript, A02135) to nucleocapsid protein of SARS-CoV-2 omicron variant.

    SARS-CoV-2 Nucleocapsid Antibody (N338) captured on Protein A sensors surface can bind omicron variant with dissociation constant (KD) of 5.56E-09M.


Background
Target Background SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2), also known as 2019-nCoV, is a positive-sense single-stranded RNA virus. It caused coronavirus disease 2019 (COVID-19). Nucleocapsid Protein is a most abundant structure protein of the coronavirus which is associated with nucleic acid.
Synonyms Mouse Anti-COVID19 N Protein Antibody; Mouse Anti-SARS-CoV-2 NP Antibody; Sars/sars-CoV-2 Coronavirus Nucleocapsid Monoclonal Antibody

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


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