Catalog Products » Estrogen receptor Antibody, mAb, Rabbit
Estrogen Receptor Antibody, MAb, Rabbit

Western blot analysis of ER alpha expression in (1) MCF7 cell lysate; (2)T47-D cell lysate.

Estrogen receptor Antibody, mAb, Rabbit

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.
A02450
$210.00

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Overview
Specificity This product is specific to Estrogen receptor
Host Species Rabbit
Immunogen A synthesized peptide derived from human Estrogen receptor
Species Reactivity Human; Mouse; Rat; Dog
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunohistochemistry (IHC) 1:100-1:200
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunohistochemistry Frozen (IHF) 1:50-1:200
Immunoprecipitation (IP) 1:20-1:50
Chromatin immunoprecipitation (ChIP) 1:20-1:50
Flow Cytometry 1:20-1:100

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal

Examples
  • Estrogen Receptor Antibody, MAb, Rabbit
    • Estrogen Receptor Antibody, MAb, Rabbit

    Western blot analysis of ER alpha expression in (1) MCF7 cell lysate; (2)T47-D cell lysate.


Background
Target Background Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.
Synonyms ER; ESR; ESR1; Era; ESRA; NR3A1;

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.