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Ku80 Antibody, mAb, Rabbit

Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA. Required for double-strand break repair and V(D)J recombination .
$210.00
A03025-50

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Overview
Specificity This product is specific to Ku80
Host Species Rabbit
Immunogen A synthesized peptide derived from human Ku80
Species Reactivity Human
Conjugate Unconjugated
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Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:5000
Immunohistochemistry (IHC) 1:100-1:200
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunohistochemistry Frozen (IHF) 1:50-1:200
Immunoprecipitation (IP) 1:20-1:50
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Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
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Ku80 Antibody, MAb, Rabbit

Western blot analysis of Ku80 expression in HeLa cell lysate. »

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Background
Target Background Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA. Required for double-strand break repair and V(D)J recombination .
Synonyms CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; G22P2; KARP1; Ku autoantigen, 80kDa; Ku80; Ku86 autoantigen related protein 1; KUB2; NFIV;
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.