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Catalog Products » Other Products » Rabbit Anti-Mouse IgM (H&L),pAb

Rabbit Anti-Mouse IgM (H&L),pAb

Description Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum.
Host Species Rabbit
Antigen Species Mouse
Conjugation Unconjugated
Immunogen Mouse IgM whole molecule

Subclass Antiserum
Concentration Lyophilized , 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity This product was prepared from monospecific antiserum by a delipidation and defibrination.  Assay by immunoelectrophoresis resulted in predominantly a single precipitin arc against Mouse IgM and Mouse Serum.  No reaction was observed against Mouse IgG F(c).  Some light chain cross reactivity may occur against Mouse IgG.
Storage Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

ELISA: 1:20,000 - 1:100,000
Immunohistochemistry: 1:1,000 - 1:5,000
Western Blot: 1:2,000 - 1:10,000
Other Dilution: User Optimized




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Cat. No. A10002
Size
Price
2 mL $110.00
24 hour
 
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