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Catalog Products » Other Products » Rabbit Anti-Mouse IgM (H&L),pAb

Rabbit Anti-Mouse IgM (H&L),pAb

Description Mouse IgM antibody recognizes the mu chain of the Mouse IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum, Mouse IgM and Mouse Serum. No reaction was observed against Mouse IgA or Mouse IgG. ELISA was used to confirm minimal cross reactivity against other mouse heavy or light chain isotypes. Anti-Mouse IgM antibody is ideal with investigators involved in serum component protein research.
Host Species Rabbit
Antigen Species Mouse
Conjugation Unconjugated
Immunogen Mouse IgM whole molecule

Subclass IgG
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity Anti-MOUSE IgM (H&L) (RABBIT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Mouse IgM.  No reaction was observed against Mouse IgG F(c).  Some light chain cross reactivity may occur against Mouse IgG.
Storage Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

ELISA: 1:20,000 - 1:100,000
Immunohistochemistry: 1:1,000 - 1:5,000
Western Blot: 1:2,000 - 1:10,000
Other Dilution: User Optimized




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Cat. No. A10005
Size
Price
50 mg $223.00
24 hour
 
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