|Description||Goat Anti-Human IgG IgA IgM antibody Peroxidase (Horseradish) conjugated, pAb. Anti-Human IgG IgA IgM (H&L) antibody generated in goat detects specifically human IgG IgA IgM (H&L). This secondary antibody anti-Human is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.|
|Immunogen||Anti-Human IgG IgA IgM (H&L) was produced by repeated immunization with Human IgG, IgA and IgM whole molecules in goat.|
|Concentration||Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Reconstitution||Restore with deionized water (or equivalent)|
|Specificity||This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG, Human IgA and Human IgM. This reagent is suitable for the detection of all Human immunoglobulin subclasses and chain combinations.|
|Storage||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|ELISA: 1:20,000 - 1:100,000
Immunohistochemistry: 1:1,000 - 1:5,000
Immunoprecipitation: 1:1,000 - 1:5,000
Western Blot: 1:2,000 - 1:10,000
Other Dilution: User Optimized
Western Blot of Goat anti-Human IgG, IgA, IgM Peroxidase Conjugated Antibody. Lane 1: Human IgG. Lane 2: Human IgA.Lane 3: Human IgM. Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min RT.Predicted/Observed size: Appropriate banding patern for Human IgG, IgA, and IgM is shown. Other band(s): Splice variants and isoforms.
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