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Catalog Products » Other Products » Goat Anti-Human IgG IgA IgM [HRP],pAb

Goat Anti-Human IgG IgA IgM [HRP],pAb

Description Anti-Human IgG IgA IgM (H&L) antibody generated in goat detects specifically human IgG IgA IgM (H&L). This secondary antibody anti-Human is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Host Species Goat
Antigen Species Human
Conjugation Peroxidase (Horseradish)
Immunogen Anti-Human IgG IgA IgM (H&L) was produced by repeated immunization with Human IgG, IgA and IgM whole molecules in goat.

Subclass IgG
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG, Human IgA and Human IgM.  This reagent is suitable for the detection of all Human immunoglobulin subclasses and chain combinations.
Storage Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

ELISA: 1:20,000 - 1:100,000
Immunohistochemistry: 1:1,000 - 1:5,000
Immunoprecipitation: 1:1,000 - 1:5,000
Western Blot: 1:2,000 - 1:10,000
Other Dilution: User Optimized

Goat Anti-Human IgG IgA IgM [HRP],pAb
Goat Anti-Human IgG IgA IgM [HRP],pAb

Western Blot of Goat anti-Human IgG, IgA, IgM Peroxidase Conjugated Antibody. Lane 1: Human IgG. Lane 2: Human IgA.Lane 3: Human IgM. Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min RT.Predicted/Observed size: Appropriate banding patern for Human IgG, IgA, and IgM is shown. Other band(s): Splice variants and isoforms.




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Cat. No. A10025
Size
Price
2 mg $178.00
24 hour
 
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