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Western Blot of Goat anti-Human IgG, IgA, IgM Peroxidase Conjugated Antibody. Lane 1: Human IgG. Lane 2: Human IgA.Lane 3: Human IgM. Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min RT.Predicted/Observed size: Appropriate banding patern for Human IgG, IgA, and IgM is shown. Other band(s): Splice variants and isoforms.
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