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Catalog Products » Other Products » Goat Anti-Human IgG IgA IgM [ALP],pAb

Goat Anti-Human IgG IgA IgM [ALP],pAb

Description Goat Anti-Human IgG IgA IgM antibody Alkaline Phosphatase (Calf Intestine) conjugated, pAb.
Host Species Goat
Antigen Species Human
Conjugation Alkaline Phosphatase (Calf Intestine)
Immunogen Human IgG, IgA and IgM whole molecule

Subclass IgG
Concentration Liquid (sterile filtered) , 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Goat Serum.  This reagent is suitable for the detection of all human immunglobulin isotypes, subclasses and chain combinations.
Storage Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

ELISA: 1:2,000 - 1:10,000
Immunohistochemistry: 1:200 - 1:1,000
Western Blot: 1:500 - 1:3,000
Other Dilution: User Optimized

Goat Anti-Human IgG IgA IgM [ALP],pAb
Goat Anti-Human IgG IgA IgM [ALP],pAb

Dot Blot of Goat anti-Human IgG IgA IgM Antibody Alkaline Phosphatase Conjugated. Antigen: Row 1 - Human IgG Row 2 - Human IgA Row 3 - Human IgM. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.Primary antibody: n/a.Secondary antibody: Goat anti-Human IgG IgA IgM Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 1 HR at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 1 HR at RT.




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Cat. No. A10027
Size
Price
1 mg $191.00
 
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