|Description||Goat Anti-Human IgG IgA IgM antibody Alkaline Phosphatase (Calf Intestine) conjugated, pAb.|
|Conjugation||Alkaline Phosphatase (Calf Intestine)|
|Immunogen||Human IgG, IgA and IgM whole molecule|
|Concentration||Liquid (sterile filtered) , 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0|
|Specificity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Goat Serum. This reagent is suitable for the detection of all human immunglobulin isotypes, subclasses and chain combinations.|
|Storage||Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.|
|ELISA: 1:2,000 - 1:10,000
Immunohistochemistry: 1:200 - 1:1,000
Western Blot: 1:500 - 1:3,000
Other Dilution: User Optimized
Dot Blot of Goat anti-Human IgG IgA IgM Antibody Alkaline Phosphatase Conjugated. Antigen: Row 1 - Human IgG Row 2 - Human IgA Row 3 - Human IgM. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.Primary antibody: n/a.Secondary antibody: Goat anti-Human IgG IgA IgM Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 1 HR at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 1 HR at RT.
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