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Home » Gene Synthesis & Molecular Biology Services » Goat Anti-Human IgG F(c) [BAC],pAb

Goat Anti-Human IgG F(c) [BAC],pAb

Description Goat Anti-Human IgG F(c) antibody Biotin conjugated, pAb.
Host Species Goat
Antigen Species Human
Conjugation Biotin
Immunogen Human IgG F(c) fragment

Subclass IgG
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum.  No reaction was observed against Human IgG F(ab)or Mouse serum proteins.
Storage Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

ELISA: 1:10,000 - 1:50,000
Immunohistochemistry: 1:500 - 1:2,500
Western Blot: 1:1,000 - 1:5,000
Other Dilution: User Optimized

  • Goat Anti-Human IgG F(c) [BAC],pAb
  • Goat Anti-Human IgG F(c) [BAC],pAb

    ELISA results of purified Goat anti-Human IgG F(c) Antibody Biotin conjugated tested against purified Human IgG F(c). Each well was coated in duplicate with 1.0 µg of Human IgG F(c). The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody.Assay performed using 3% fish gelatin as blocking buffer, Streptavidin Peroxidase Conjugated and TMB substrate.

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Cat. No. A10028
1 mg $129.00