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Catalog Products » Other Products » Goat Anti-Human IgG (H&L) [BAC],pAb

Goat Anti-Human IgG (H&L) [BAC],pAb

Description Secondary Antibodies are ideal for western blot and are available in a variety of formats and conjugate types. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment compositionMin X Bv Ch Gt GP Ham Hs Ms Rb Rt & Sh Serum Proteins
Host Species Goat
Antigen Species Human
Conjugation Biotin
Immunogen Human IgG whole molecule

Subclass IgG
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity Secondary antibody conjugate was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Human IgG and Human Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins.
Species Reactivity Human
Storage Store Secondary Antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

ELISA: 1:20,000 - 1:100,000
Immunohistochemistry: 1:1,000 - 1:5,000
Western Blot: 1:2,000 - 1:10,000
Other Dilution: User Optimized

Goat Anti-Human IgG (H&L) [BAC],pAb
Goat Anti-Human IgG (H&L) [BAC],pAb

Western Blot of Goat anti-Human IgG Pre-Adsorbed Biotin Conjugated Secondary Antibody. Lane 1: Human IgG. Lane 2: None. Load: 50 ng per lane.Primary antibody: Biotin conjugated goat anti-Human IgG 1:1,000 for 60 min at RT.Secondary antibody: Peroxidase Streptavidin secondary antibody at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Human IgG. Other band(s): None.




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Cat. No. A10029
Size
Price
1 mg $176.00
24 hour
 
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