|Description||Goat Anti-Human λ chain antibody Unconjugated, pAb. Anti-Human lambda light chain antibody generated in goat detects specifically human lambda light chain . This secondary antibody anti-Human is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.|
|Immunogen||Anti-Human lambda light chain antibody was produced by repeated immunization with Human lambda light chain molecule in goat.|
|Concentration||Liquid (sterile filtered) , 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0|
|Specificity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. Solid phase adsorption(s) against a panel of Human IgG Kappa, IgM Kappa and IgA Kappa proteins was performed. Specificity was confirmed by ELISA.|
|Storage||Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.|
|ELISA: 1:4,000 - 1:20,000
Immunohistochemistry: 1:1,000 - 1:5,000
Western Blot: 1:500 - 1:2,000
Other Dilution: User Optimized
Western Blot of Goat anti-Human λ (lambda chain) Antibody.Lane 1: Human λ (lambda chain).Lane 2: None.Load: 100 ng per lane.Primary antibody: Human λ (lambda chain) Antibody at 1:1000 for overnight at 4°C.Secondary antibody: DyLight800 Donkey anti-Goat at 1:20,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Other Band(s): None.
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