|Description||Goat Anti-Human IgG (H&L) antibody Peroxidase (Horseradish) conjugated, pAb. Anti-Human IgG (H&L) peroxidase conjugated antibody generated in goat detects specifically human IgG. This secondary peroxidase conjugated antibody anti-Human is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.|
|Immunogen||Anti-Human IgG (H&L) was produced by repeated immunization with human IgG whole molecule in goat.|
|Concentration||Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Reconstitution||Restore with deionized water (or equivalent)|
|Specificity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG and Human Serum.|
|Storage||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|ELISA: 1:20,000 - 1:80,000
Immunohistochemistry: 1:1,000 - 1:5,000
Western Blot: 1:5,000 - 1:25,000
Other Dilution: User Optimized
ELISA results of purified Goat anti-Human IgG Antibody Peroxidase conjugated tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 1.0 µg of Human IgG. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.
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