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Goat Anti-Human κ chain [BAC],pAb

Description Goat Anti-Human κ chain antibody Biotin conjugated, pAb. Human k (kappa chain) detects the kappa light chain of human immunoblogulin.
Host Species Goat
Antigen Species Human
Conjugation Biotin
Immunogen Human kappa light chain
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Subclass IgG
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity Anti-Human k was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Goat Serum.  Specificity was confirmed by ELISA minimal against other human heavy or light chain proteins.
Storage Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

ELISA: 1:200,000
Immunohistochemistry: 1:1,000 - 1:5,000
Western Blot: 1:2,000 - 1:10,000
Other Dilution: User Optimized

  • Goat Anti-Human κ Chain [BAC],pAb
  • Goat Anti-Human κ Chain [BAC],pAb

    Western blot of Biotin conjugated Goat Anti-Human κ secondary antibody.Lane 1: Human κ.Lane 2: Human λ.Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Biotin rabbit secondary antibody at 1:1,000 for 60 min at RT.Blocking: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 28 kDa, 28 kDa for Human κ.Other band(s): None.

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Cat. No. A10045
1 mg $178.00