|Description||Mouse Anti-Human IgG (H&L) antibody Unconjugated, pAb.|
|Immunogen||Human IgG whole molecule|
|Concentration||Liquid (sterile filtered) , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Specificity||This product was prepared from monospecific polyclonal ascites by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgG and Human Serum.|
|Storage||Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.|
|ELISA: 1:5,000 - 1:50,000
Immunohistochemistry: 1:1,000 - 1:5,000
Western Blot: 1:500 - 1:5,000
Other Dilution: User Optimized
Western Blot of Human IgG Antibody.M: 3 µL Opal Prestained Ladder (MB-210-0500).Lane 1: Human IgG. Load: 50 ng per lane.Primary antibody: Human IgG Antibody at 1:1,000 overnight at 4°C.Secondary antibody: Peroxidase conjugated mouse secondary antibody at 1:40,000 for 30 minutes at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 minutes at RT.Predicted/Observed size: 55 kDa, 25 kDa for Human IgG.
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