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Western Blot of Rabbit Anti-HUMAN IgA (alpha chain) Antibody Lane 1: Human IgALoad: 100 ng per lanePrimary antibody: HUMAN IgA (alpha chain) Antibody at 1:1000 for 60 min at RTSecondary antibody: DyLight 649 goat anti-rabbit at 1:20,000 for 30 min at RTBlock: 5% BLOTTO 30 min at RTPredicted/Observed size: 60and 28 kDa, 60 and 28 kDa
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