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Catalog Products » Other Products » Rabbit Anti-Human IgA,pAb

Rabbit Anti-Human IgA,pAb

Description Rabbit Anti-Human IgA antibody Unconjugated, pAb.
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen Human IgA alpha heavy chain

Subclass IgG
Concentration Liquid (sterile filtered) , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgA coupled to agarose followed by solid phase adsorption(s).  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgA and Human Serum.  No reaction was observed against other human heavy or light chain proteins.
Storage Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

ELISA: 1:5,000 - 1:20,000
Immunohistochemistry: 1:250 - 1:1,000
Western Blot: 1:500 - 1:2,000
Other Dilution: User Optimized

Rabbit Anti-Human IgA,pAb
Rabbit Anti-Human IgA,pAb

Western Blot of Rabbit Anti-HUMAN IgA (alpha chain) Antibody Lane 1: Human IgALoad: 100 ng per lanePrimary antibody: HUMAN IgA (alpha chain) Antibody at 1:1000 for 60 min at RTSecondary antibody: DyLight 649 goat anti-rabbit at 1:20,000 for 30 min at RTBlock: 5% BLOTTO 30 min at RTPredicted/Observed size: 60and 28 kDa, 60 and 28 kDa




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Cat. No. A10050
Size
Price
2 mg $124.00
24 hour
 
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