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Catalog Products » Other Products » Rabbit Anti-Human IgG (H&L) [HRP],pAb

Rabbit Anti-Human IgG (H&L) [HRP],pAb

Description Anti-Human IgG whole molecule antibody generated in rabbit detects specifically human IgG whole molecule. This secondary antibody anti-Human is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Host Species Rabbit
Antigen Species Human
Conjugation Peroxidase (Horseradish)
Immunogen Anti-Human IgG whole molecule was produced by repeated immunization with Human IgG whole molecule in rabbit.

Subclass IgG
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgG and Human Serum.
Species Reactivity Human
Storage Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

ELISA: 1:150,000 - 1:250,000
Immunohistochemistry: 1:1,000 - 1:5,000
Western Blot: 1:5,000 - 1:25,000
Other Dilution: User Optimized

Rabbit Anti-Human IgG (H&L) [HRP],pAb
Rabbit Anti-Human IgG (H&L) [HRP],pAb

ELISA results of Rabbit anti-Human IgG Antibody Peroxidase Conjugated tested against purified Human IgG protein. Each well was coated in duplicate with 1.0 µg of Human IgG. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.




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Cat. No. A10053
Size
Price
2 mg $129.00
24 hour
 
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