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Rabbit Anti-Human IgG F(c) [HRP],pAb

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Overview

Description	Rabbit Anti-Human IgG F(c) antibody Peroxidase (Horseradish) conjugated, pAb.
Host Species	Rabbit
Antigen Species	Human
Conjugation	Peroxidase (Horseradish)
Immunogen	Human IgG F(c) fragment

Properties

Subclass	IgG
Concentration	Lyophilized , 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution	Restore with deionized water (or equivalent)
Specificity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against Human IgG F(ab)
Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Applications

ELISA: 1:10,000 - 1:50,000
Immunohistochemistry: 1:500 - 1:2,500
Western Blot: 1:1,000 - 1:5,000
Other Dilution: User Optimized

ELISA results of purified Rabbit anti-Human IgG F(c) Antibody Peroxidase Conjugated tested against purified Human IgG F(c). Each well was coated in duplicate with 1.0 µg of Human IgG F(c). The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.