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Rabbit Anti-Human IgG (H&L) [ALP],pAb

Description Rabbit Anti-Human IgG (H&L) antibody Alkaline Phosphatase (Calf Intestine) conjugated, pAb. Human IgG antibody detects human IgG whole molecule. Anti-Human IgG antibody is ideal for investigators involved in serum protein component research.
Host Species Rabbit
Antigen Species Human
Conjugation Alkaline Phosphatase (Calf Intestine)
Immunogen Human IgG whole molecule

Subclass IgG
Concentration Liquid (sterile filtered) , 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Specificity Anti-HUMAN IgG antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Human IgG and Human Serum.
Storage Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

ELISA: 1:2,000 - 1:10,000
Immunohistochemistry: 1:200 - 1:1,000
Western Blot: 1:500 - 1:2,500
Other Dilution: User Optimized

  • Rabbit Anti-Human IgG (H&L) [ALP],pAb
  • Rabbit Anti-Human IgG (H&L) [ALP],pAb

    Dot Blot of Rabbit anti-Human IgG Antibody Alkaline Phosphatase Conjugated. Antigen: Human IgG.Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.Primary antibody: n/a.Secondary antibody: Rabbit anti-Human IgG Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 1 HR at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 1 HR at RT.

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Cat. No. A10059
1 mg $153.00