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Home » Gene Synthesis & Molecular Biology Services » Rabbit Anti-Human IgG F(c) [ALP],pAb

Rabbit Anti-Human IgG F(c) [ALP],pAb

*This product has been discontinued!*
Description Rabbit Anti-Human IgG F(c) antibody Alkaline Phosphatase (Calf Intestine) conjugated, pAb. Human IgG F(c) antibody generated in rabbit detects specifically Anti-Human IgG F(c) fragment. This secondary antibody anti-Human IgG F(c) is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Host Species Rabbit
Antigen Species Human
Conjugation Alkaline Phosphatase (Calf Intestine)
Immunogen Anti-Human IgG F(c) was produced by repeated immunization with Human Fc fragment in rabbit.

Subclass IgG
Concentration Liquid (sterile filtered) , 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Specificity Anti-Human IgG F(c) was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Human IgG, Human IgG F(c) and Human Serum.  No reaction was observed against Human IgG F(ab).
Species Reactivity Human
Storage Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

ELISA: 1:2,000 - 1:10,000
Immunohistochemistry: 1:200 - 1:1,000
Western Blot: 1:500 - 1:2,500
Other Dilution: User Optimized




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