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Rabbit Anti-Human IgG F(c) [ALP],pAb

Description Rabbit Anti-Human IgG F(c) antibody Alkaline Phosphatase (Calf Intestine) conjugated, pAb. Human IgG F(c) antibody generated in rabbit detects specifically Anti-Human IgG F(c) fragment. This secondary antibody anti-Human IgG F(c) is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Host Species Rabbit
Antigen Species Human
Conjugation Alkaline Phosphatase (Calf Intestine)
Immunogen Anti-Human IgG F(c) was produced by repeated immunization with Human Fc fragment in rabbit.

Subclass IgG
Concentration Liquid (sterile filtered) , 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Specificity Anti-Human IgG F(c) was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Human IgG, Human IgG F(c) and Human Serum.  No reaction was observed against Human IgG F(ab).
Species Reactivity Human
Storage Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

ELISA: 1:2,000 - 1:10,000
Immunohistochemistry: 1:200 - 1:1,000
Western Blot: 1:500 - 1:2,500
Other Dilution: User Optimized

  • Rabbit Anti-Human IgG F(c) [ALP],pAb
  • Rabbit Anti-Human IgG F(c) [ALP],pAb

    Dot Blot of Human IgG Fc Antibody Alkaline Phosphatase Conjugated.Antigen: Human IgG Fc Load: Lane 1 - 100ng Lane 2 - 33.33ng Lane 3 - 11.11ng Lane 4 - 3.70ng Lane 5 - 1.23ngPrimary antibody: n/aSecondary antibody: Human IgG Fc Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 60 min at RT.Visualized using Alkaline Phosphatase Substrate.

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Cat. No. A10060
1 mg $181.00