×
Catalog Products » Other Products » Goat Anti-Mouse IgG (H&L) [TRITC],pAb

Goat Anti-Mouse IgG (H&L) [TRITC],pAb

Description This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Host Species Goat
Antigen Species Mouse
Conjugation Rhodamine (TRITC)
Immunogen Mouse IgG whole molecule

Subclass IgG
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum.
Species Reactivity Mouse
Storage Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.


FLISA: 1:10,000 - 1:50,000
Flow Cytometry: 1:500 - 1:2,500
IF Microscopy: 1:1,000 - 1:5,000
Other Dilution: User Optimized

Goat Anti-Mouse IgG (H&L) [TRITC],pAb
Goat Anti-Mouse IgG (H&L) [TRITC],pAb

Western Blot of Goat anti-Rabbit IgG Peroxidase Conjugated Antibody. Lane 1: HeLa Whole Cell Lysate. Lane 2: NIH 3T3 Whole Cell Lysate. Load: 10 µg per lane.Primary antibody: Beta Actin antibody at 1:2,000 for overnight at 4°C.Secondary antibody (2 Blots on Left): Atto 647N goat secondary antibody at 1:10,000 for 60 min at RT.Secondary Antibody (2 Blots on Right): Peroxidase goat secondary antibody at 1:10,000 for 60 min at RT.Block: 5% BLOTTO overnight at 4°C.Predicted/Observed size: 50 kDa, 50 kDa for Beta Actin. Other band(s): none.




Notice there is no results found, you can visit "Citations Database" for other results.







For more documents, please visit "Technical Support".

Cat. No. A10072
Size
Price
2 mg $105.00
24 hour
 
*
*
*
*