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Dot Blot showing the detection of Mouse IgG. A three-fold serial dilution of Mouse IgG starting at 200ng was spotted onto 0.45 ?m nitrocellulose. After blocking in 5% Blotto 1 Hour at 20°C, Anti-Mouse IgG (H&L) (GOAT) Antibody Fluorescein Conjugated (Cat. No. A10076) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting and imaged using the Bio-Rad VersaDoc® 4000 MP.
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