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Goat Anti-Mouse IgG (H&L) [FITC],pAb

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Overview

Description	Goat Anti-Mouse IgG (H&L) antibody Fluorescein (FITC) conjugated, pAb. FITC Conjugated Secondary Antibodies are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins
Host Species	Goat
Antigen Species	Mouse
Conjugation	Fluorescein (FITC)
Immunogen	Mouse IgG whole molecule

Properties

Subclass	IgG
Concentration	Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution	Restore with deionized water (or equivalent)
Specificity	Secondary antibody to Mouse IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
Species Reactivity	Mouse
Storage	Store anti mouse secondary antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Applications

FLISA: 1:10,000 - 1:50,000
Flow Cytometry: 1:200 - 1:500
IF Microscopy: 1:1,000 - 1:5,000
Other Dilution: User Optimized

Dot Blot showing the detection of Mouse IgG. A three-fold serial dilution of Mouse IgG starting at 200ng was spotted onto 0.45 ?m nitrocellulose. After blocking in 5% Blotto 1 Hour at 20°C, Anti-Mouse IgG (H&L) (GOAT) Antibody Fluorescein Conjugated (Cat. No. A10076) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting and imaged using the Bio-Rad VersaDoc® 4000 MP.