|Description||Goat Anti-Mouse IgG (H&L) antibody Alkaline Phosphatase (Calf Intestine) conjugated, pAb. Conjugated Secondary Antibodies are ideal for western blot and are available in a variety of formats and conjugate types. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins|
|Conjugation||Alkaline Phosphatase (Calf Intestine)|
|Immunogen||Mouse IgG whole molecule|
|Concentration||Liquid (sterile filtered) , 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0|
|Specificity||Mouse Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.|
|Storage||Store secondary antibody at 4° C before opening. DO NOT FREEZE. Conjugated antibody is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.|
|ELISA: 1:2,000 - 1:10,000
Immunohistochemistry: 1:200 - 1:1,000
Western Blot: 1:500 - 1:2,500
Other Dilution: User Optimized
Dot Blot of Goat anti-Mouse IgG antibody Alkaline Phosphatase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins. Antigen: Mouse IgG. Load: 200 ng, 66.7 ng, 22.2 ng, 7.41 ng, or 2.47 ng as indicated.Primary antibody: Anti-Mouse IgG antibody Alkaline Phosphatase Conjugatedat 1:1,000 for 60 min at 4°C.Secondary antibody: N/A.Block: Blocking Buffer for Fluorescent Western Blotting (Blocking Buffer for Fluorescent Western Blotting) for 60 min at RT.
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