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Catalog Products » Other Products » Goat Anti-Mouse IgG (H&L) [RPE],pAb

Goat Anti-Mouse IgG (H&L) [RPE],pAb

Description Anti-Mouse IgG (H&L) Phycoerythrin conjugated antibody generated in goat detects specifically mouse IgG (H&L). This secondary conjugated antibody anti-mouse is ideal for investigators who routinely perform immunomicroscopy and flow cytometry or FACS analysis as well as other antibody-based fluorescent assays requiring extremely low background levels.
Host Species Goat
Antigen Species Mouse
Conjugation R-Phycoerythrin (RPE)
Immunogen Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in goat.

Subclass IgG
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, conjugation to phycoerythrin and subsequent purification, and extensive dialysis against the buffer stated above.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Mouse IgG and Mouse Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
Species Reactivity Mouse
Storage Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.


Flow Cytometry: 1:100 - 1:250
IF Microscopy: 1:100 - 1:250
Other Dilution: User Optimized

Goat Anti-Mouse IgG (H&L) [RPE],pAb
Goat Anti-Mouse IgG (H&L) [RPE],pAb

Dot Blot of Goat anti-Mouse IgG Antibody Phycoerythrin Conjugated. Antigen: Mouse IgG. Load: 3-fold serial dilution starting at 200 ng.Primary antibody: None.Secondary antibody: Phycoerythrin goat secondary antibody at 1:1,000 for 60 min at RT.Block: 5% BLOTTO 1 Hour at RT.




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Cat. No. A10085
Size
Price
1 mL $216.00
24 hour
 
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