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Western Blot of Goat anti-Mouse IgG (H&L) Antibody.Lane 1: Mouse IgG.Lane 2: None.Load: 100 ng per lane.Primary Antibody: Mouse IgG (H&L) Antibody 1:1000 overnight at 4°C.Secondary antibody: DyLight™ 800 goat secondary antibody at 1:20,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Obsevered Size: 28 and 55 kDa/28 and 55 kDa for Mouse IgG.Other band(s): none.
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