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Goat Anti-Mouse IgG (H&L),pAb

Description Goat Anti-Mouse IgG (H&L) antibody Unconjugated, pAb. Anti-Mouse IgG affinity purified secondary antibody is generated in goat detects specifically Mouse IgG whole molecule . This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Host Species Goat
Antigen Species Mouse
Conjugation Unconjugated
Immunogen Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in goat.

Subclass IgG
Concentration Liquid (sterile filtered) , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Specificity Secondary Antibody Anti-Mouse IgG (H&L) was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum.
Species Reactivity Mouse
Storage Store Anti-Mouse Secondary Antibody at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

ELISA: 1:25,000
Flow Cytometry: Yes
Immunohistochemistry: 1:1,000 - 1:5,000
IF Microscopy: Yes
Immunoprecipitation: Yes
Western Blot: 1:3,000 - 1:15,000
Other Dilut

  • Goat Anti-Mouse IgG (H&L),pAb
  • Goat Anti-Mouse IgG (H&L),pAb

    Western Blot of Goat anti-Mouse IgG (H&L) Antibody.Lane 1: Mouse IgG.Lane 2: None.Load: 100 ng per lane.Primary Antibody: Mouse IgG (H&L) Antibody 1:1000 overnight at 4°C.Secondary antibody: DyLight™ 800 goat secondary antibody at 1:20,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Obsevered Size: 28 and 55 kDa/28 and 55 kDa for Mouse IgG.Other band(s): none.

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Cat. No. A10087
2 mg $95.00