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ELISA results of Goat anti-Mouse IgG F(c) Antibody tested against purified Mouse IgG F(c) protein. Each well was coated in duplicate with 1.0 µg of Mouse IgG F(c). The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Donkey anti-Goat IgG Antibody Peroxidase Conjugated (Min X Ch GP Ham Hs Ms Rb & Rt Serum Proteins) (Cat. No. A10015) and TMB substrate.
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