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Catalog Products » Other Products » Goat Anti-Mouse IgG (H&L) [HRP],pAb

Goat Anti-Mouse IgG (H&L) [HRP],pAb

Description Anti-Mouse IgG whole molecule antibody generated in goat detects specifically Mouse IgG whole molecule . This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Host Species Goat
Antigen Species Mouse
Conjugation Peroxidase (Horseradish)
Immunogen Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in goat.

Subclass IgG
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity MOUSE IgG (H&L) Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum.
Species Reactivity Mouse
Storage Store HRP Conjugated Mouse Secondary Antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

ELISA: 1:40,000 - 1:80,000
Immunohistochemistry: 1:500 - 1:2,500
Western Blot: 1:2,000 - 1:10,000
Other Dilution: User Optimized

Goat Anti-Mouse IgG (H&L) [HRP],pAb
Goat Anti-Mouse IgG (H&L) [HRP],pAb

Western Blot of Peroxidase conjugated Goat anti-Mouse IgG antibody. Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane.Primary antibody: none.Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting 30 min at RT.Predicted/Observed size: 50 kDa, 25 kDa for Mouse IgG. Other band(s): none.




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Cat. No. A10093
Size
Price
2 mg $129.00
 
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