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Catalog Products » Other Products » Goat Anti-Mouse IgG F(c) [HRP],pAb

Goat Anti-Mouse IgG F(c) [HRP],pAb

Description Anti-Mouse IgG F(c) fragment antibody generated in goat detects specifically Mouse IgG F(c) fragment. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Host Species Goat
Antigen Species Mouse
Conjugation Peroxidase (Horseradish)
Immunogen Anti-Mouse IgG F(c) fragment was produced by repeated immunization with Mouse IgG F(c) fragment in goat.

Subclass IgG
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum.  No reaction was observed against Mouse IgG F(ab')2 fragment.
Storage Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

ELISA: 1:10,000 - 1:50,000
Immunohistochemistry: 1:500 - 1:2,500
Western Blot: 1:1,000 - 1:5,000
Other Dilution: User Optimized

Goat Anti-Mouse IgG F(c) [HRP],pAb
Goat Anti-Mouse IgG F(c) [HRP],pAb

Western blot of Peroxidase conjugated Goat Anti-Mouse IgG F(c) secondary antibody.Lane 1: Mouse IgG F(c).Lane 2: None.Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT.Blocking: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 28 kDa, 31 kDa for Mouse IgG F(c).Other band(s): None.




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Cat. No. A10094
Size
Price
2 mg $162.00
24 hour
 
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