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Western Blot of Alkaline Phosphatase Conjugated Goat Anti-Mouse IgG Antibody Lane 1: Mouse IgGLoad: 100 ng per laneSecondary antibody: Alkaline Phosphatase Conjugated Goat Anti-Mouse IgG Antibody at 1:1000 for 60 min at RTBlock: Blocking Buffer for Fluorescent Western Blotting for 30 min RTPredicted/Observed size: 55 and 28 kDa, 55 and 28 kDa
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