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Goat Anti-Mouse IgG (H&L) [ALP],pAb

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Overview

Description	Goat Anti-Mouse IgG (H&L) antibody Alkaline Phosphatase (Calf Intestine) conjugated, pAb. Mouse secondary antibody conjugated to Alkaline Phosphatase is available in a variety of formats. Anti IgG secondary antibody conjugate is suitable for ELISA, Immunohistochemistry, Western Blotting as well as other Alkaline Phosphatase antibody based assays.
Host Species	Goat
Antigen Species	Mouse
Conjugation	Alkaline Phosphatase (Calf Intestine)
Immunogen	Mouse IgG whole molecule

Properties

Subclass	IgG
Concentration	Liquid (sterile filtered) , 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Specificity	Anti-Mouse Conjugated Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Mouse IgG and Mouse Serum.
Storage	Store secondary antibody conjugate at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Applications

ELISA: 1:15,000
Immunohistochemistry: 1:200 - 1:1,000
Western Blot: 1:1,000 - 1:4,000
Other Dilution: User Optimized

Western Blot of Alkaline Phosphatase Conjugated Goat Anti-Mouse IgG Antibody Lane 1: Mouse IgGLoad: 100 ng per laneSecondary antibody: Alkaline Phosphatase Conjugated Goat Anti-Mouse IgG Antibody at 1:1000 for 60 min at RTBlock: Blocking Buffer for Fluorescent Western Blotting for 30 min RTPredicted/Observed size: 55 and 28 kDa, 55 and 28 kDa