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Catalog Products » Other Products » Goat Anti-Mouse IgG (H&L) [BAC],pAb

Goat Anti-Mouse IgG (H&L) [BAC],pAb

Description Anti-Mouse IgG (H&L) biotin conjugated antibody generated in goat detects specifically mouse IgG (H&L). This secondary conjugated antibody anti-mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Host Species Goat
Antigen Species Mouse
Conjugation Biotin
Immunogen Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in goat.

Subclass IgG
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Mouse IgG and Mouse Serum.
Species Reactivity Mouse
Storage Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

ELISA: 1:350,000
Immunohistochemistry: 1:1,000 - 1:5,000
Western Blot: 1:2,000 - 1:20,000
Other Dilution: User Optimized

Goat Anti-Mouse IgG (H&L) [BAC],pAb
Goat Anti-Mouse IgG (H&L) [BAC],pAb

ELISA results of purified Goat anti-Mouse IgG Antibody Biotin Conjugated tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Streptavidin Peroxidase Conjugated and TMB substrate.




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Cat. No. A10099
Size
Price
2 mg $114.00
24 hour
 
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