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Catalog Products » Other Products » Rabbit Anti-Mouse IgG (H&L) [TRITC],pAb

Rabbit Anti-Mouse IgG (H&L) [TRITC],pAb

Description This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Host Species Rabbit
Antigen Species Mouse
Conjugation Rhodamine (TRITC)
Immunogen Mouse IgG whole molecule

Subclass IgG
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG, and Mouse Serum.
Storage Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.


FLISA: 1:10,000 - 1:50,000
Flow Cytometry: 1:500 - 1:2,500
IF Microscopy: 1:1,000 - 1:5,000
Other Dilution: User Optimized

Rabbit Anti-Mouse IgG (H&L) [TRITC],pAb
Rabbit Anti-Mouse IgG (H&L) [TRITC],pAb

Western blot of Rhodamine conjugated Rabbit Anti-Mouse IgG secondary antibody.Lane 1: Mouse IgG.Lane 2: None.Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Rhodamine rabbit secondary antibody at 1:1,000 for 60 min at RT.Blocking: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG.Other band(s): None.




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Cat. No. A10101
Size
Price
2 mg $110.00
24 hour
 
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