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Catalog Products » Other Products » Rabbit Anti-Mouse IgG (H&L),pAb

Rabbit Anti-Mouse IgG (H&L),pAb

Description Rabbit Anti-Mouse IgG (H&L) antibody Unconjugated, pAb. Mouse IgG Secondary Antibodies are ideal for Western Blotting, Immunohistochemistry, ELISA as well as other anibody detection methods.
Host Species Rabbit
Antigen Species Mouse
Conjugation Unconjugated
Immunogen Mouse IgG whole molecule

Subclass IgG
Concentration Liquid (sterile filtered) , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Specificity Anti-Mouse Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum.
Storage Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

ELISA: 1:20,000 - 1:400,000
Immunohistochemistry: 1:1,000 - 1:5,000
Western Blot: 1:2,000 - 1:10,000
Other Dilution: User Optimized

  • Rabbit Anti-Mouse IgG (H&L),pAb
  • Rabbit Anti-Mouse IgG (H&L),pAb

    ELISA results of purified Rabbit anti-Mouse IgG Antibody tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (Cat. No. A10160) and TMB substrate.




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Cat. No. A10104
Size
Price
2 mg $105.00
 
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