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Catalog Products » Other Products » Rabbit Anti-Mouse IgG (H&L),pAb

Rabbit Anti-Mouse IgG (H&L),pAb

Description Rabbit Anti-Mouse IgG (H&L) antibody Unconjugated, pAb. Anti-Mouse antibody generated in rabbit detects specifically mouse IgG (H&L). This secondary antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.Min X Human Serum Proteins
Host Species Rabbit
Antigen Species Mouse
Conjugation Unconjugated
Immunogen Anti-Mouse IgG was produced by repeated immunization with mouse IgG whole molecule in rabbit

Subclass IgG
Concentration Liquid (sterile filtered) , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis (IEP) resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum.  No reaction was observed against Human Serum Proteins.
Species Reactivity Mouse
Storage Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

ELISA: 1:20,000 - 1:40,000
Immunohistochemistry: 1:1,000 - 1:5,000
Western Blot: 1:2,000 - 1:10,000
Other Dilution: User Optimized

  • Rabbit Anti-Mouse IgG (H&L),pAb
  • Rabbit Anti-Mouse IgG (H&L),pAb

    ELISA results of purified Rabbit anti-Mouse IgG Antibody (min x Human Serum Proteins) tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin, Goat anti-Rabbit igG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (Cat. No. A10160) and TMB ELISA Substrate.




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Cat. No. A10110
Size
Price
2 mg $123.00
 
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