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Catalog Products » Other Products » Rabbit Anti-Mouse IgG1 [FITC],pAb

Rabbit Anti-Mouse IgG1 [FITC],pAb

Description Mouse IgG1 secondary antibody conjugated to FITC is available in a variety of formats. Anti Mouse IgG1 secondary antibody conjugate is suitable for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This IgG1 antibody is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Host Species Rabbit
Antigen Species Mouse
Conjugation Fluorescein (FITC)
Immunogen Mouse IgG1 heavy chain

Subclass IgG
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Mouse IgG and Mouse Serum.   Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
Storage Store secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.


FLISA: 1:10,000 - 1:50,000
Flow Cytometry: User Optimized
IF Microscopy: 1:500 - 1:2,500
Other Dilution: User Optimized

Rabbit Anti-Mouse IgG1 [FITC],pAb
Rabbit Anti-Mouse IgG1 [FITC],pAb

Western blot of Fluorescein conjugated Rabbit Anti-Mouse IgG1 (Gamma 1 chain) secondary antibody.Lane 1: Mouse IgG1.Lane 2: None.Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Fluorescein rabbit secondary antibody at 1:1,000 for 60 min at RT.Blocking: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG1 (Gamma 1 chain).Other band(s): None.




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Cat. No. A10116
Size
Price
1 mg $178.00
24 hour
 
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