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Catalog Products » Other Products » Rabbit Anti-Mouse IgG2a [FITC],pAb

Rabbit Anti-Mouse IgG2a [FITC],pAb

Description Rabbit Anti-Mouse IgG2a antibody Fluorescein (FITC) conjugated, pAb. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Host Species Rabbit
Antigen Species Mouse
Conjugation Fluorescein (FITC)
Immunogen Mouse IgG2a heavy chain

Subclass IgG
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG2a coupled to agarose beads followed by solid phase adsorption(s) against a panel of immobilized mouse and human serum proteins resulting in a conjugated antibody designed for flow cytometry.  Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse or human heavy or light chain isotypes.
Storage Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.


FLISA: 1:10,000 - 1:50,000
Flow Cytometry: 1:500 - 1:2,500
IF Microscopy: 1:1,000 - 1:5,000
Other Dilution: User Optimized

  • Rabbit Anti-Mouse IgG2a [FITC],pAb
  • Rabbit Anti-Mouse IgG2a [FITC],pAb

    Western blot of Fluorescein conjugated Rabbit Anti-Mouse IgG2a (Gamma 2a chain) secondary antibody.Lane 1: Mouse IgG2a.Lane 2: None.Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Fluorescein rabbit secondary antibody at 1:1,000 for 60 min at RT.Blocking: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG2a (Gamma 2a chain).Other band(s): None.




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Cat. No. A10117
Size
Price
1 mg $178.00