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Western Blot of Rabbit anti-Mouse IgG Antibody Alkaline Phosphatase ConjugatedLane 1: Mouse IgG Load: 100 ng per laneSecondary antibody: MOUSE IgG (H&L) Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RTBlock: Blocking Buffer for Fluorescent Western Blotting for 30 min at RTPredicted/Observed size: 55 and 28 kDa, 55 and 28 kDa
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