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Western Blot of Mouse IgG2a Antibody Alkaline Phosphatase Conjugated.Lane 1: Mouse IgG2a.Lane 2: none. Load: 50ng per lane.Primary antibody: none.Secondary antibody: Mouse IgG2a Antibody Alkaline Phosphatase Conjugated at 1:1,000 o/n at 4°C.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Reaction visualized with alkaline phosphatase substrate for 30 seconds at RT.Predicted/Observed size: 55kDa for Mouse IgG2a.
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