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Catalog Products » Other Products » Donkey Anti-Mouse IgG (H&L) [FITC],pAb

Donkey Anti-Mouse IgG (H&L) [FITC],pAb

Description This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins
Host Species Donkey
Antigen Species Mouse
Conjugation Fluorescein (FITC)
Immunogen Mouse IgG whole molecule

Subclass IgG
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Donkey Serum, Mouse IgG and Mouse Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
Storage Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.


Flow Cytometry: User Optimized
IF Microscopy: 1:500 - 1:2,500
Other Dilution: User Optimized

Donkey Anti-Mouse IgG (H&L) [FITC],pAb
Donkey Anti-Mouse IgG (H&L) [FITC],pAb

Western Blot of Donkey anti-Mouse IgG Pre-Absorbed Fluorescein Conjugated Secondary Antibody. Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Fluorescein donkey secondary antibody at 1:1,000 for 60 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None.




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Cat. No. A10147
Size
Price
1 mg $176.00
24 hour
 
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