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Catalog Products » Other Products » Donkey Anti-Mouse IgG (H&L) [HRP],pAb

Donkey Anti-Mouse IgG (H&L) [HRP],pAb

Description Donkey Anti-Mouse IgG (H&L) antibody Peroxidase (Horseradish) conjugated, pAb. Anti-Mouse IgG whole molecule antibody generated in donkey detects specifically Mouse IgG whole molecule . This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins
Host Species Donkey
Antigen Species Mouse
Conjugation Peroxidase (Horseradish)
Immunogen Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in donkey.
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Subclass IgG
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity Mouse IgG (H&L) Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Mouse IgG and Mouse Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins
Species Reactivity Mouse
Storage Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

ELISA: 1:20,000 - 1:80,000
Immunohistochemistry: 1:1,000 - 1:5,000
Western Blot: 1:2,000 - 1:20,000
Other Dilution: User Optimized

  • Donkey Anti-Mouse IgG (H&L) [HRP],pAb
  • Donkey Anti-Mouse IgG (H&L) [HRP],pAb

    Western Blot of Peroxidase Donkey Anti-Mouse IgG Pre-Adsorbed secondary antibody. Lane 1: Mouse IgG.Lane 2: None.Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Peroxidase donkey secondary antibody at 1:1,000 for 60 min at RT.Blocking: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG.Other band(s): None.




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Cat. No. A10149
Size
Price
1 mg $195.00
 
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