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Chicken Anti-Mouse IgG (H&L) [HRP],pAb

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Overview

Description	Chicken Anti-Mouse IgG (H&L) antibody Peroxidase (Horseradish) conjugated, pAb. Anti-Mouse (H&L) peroxidase conjugated antibody generated in chicken detects specifically mouse IgG (H&L). This secondary peroxidase conjugated antibody anti-mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Host Species	Chicken
Antigen Species	Mouse
Conjugation	Peroxidase (Horseradish)
Immunogen	Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in chicken.

Properties

Subclass	IgG
Concentration	Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution	Restore with deionized water (or equivalent)
Specificity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Chicken Serum, Mouse IgG and Mouse Serum.
Species Reactivity	Mouse
Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Applications

ELISA: 1:20,000 - 1:100,000
Immunohistochemistry: 1:500 - 1:2,500
Western Blot: 1:2,000 - 1:10,000
Other Dilution: User Optimized

Western blot of Peroxidase conjugated Chicken Anti-Mouse IgG secondary antibody.Lane 1: Mouse IgG.Lane 2: None.Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Peroxidase chicken secondary antibody at 1:1,000 for 60 min at RT.Blocking: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG.Other band(s): None.