|Description||Goat Anti-Rabbit IgG (H&L) antibody Unconjugated, pAb. Anti-Rabbit IgG antibody generated in goat detects specifically rabbit IgG. This secondary antibody anti-Rabbit is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins|
|Immunogen||Anti-Rabbit IgG (H&L) was produced by repeated immunization with rabbit whole IgG molecule in goat.|
|Concentration||Liquid (sterile filtered) , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Specificity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins. Specificity was confirmed using ELISA against at less than 1% of target signal.|
|Storage||Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.|
|ELISA: 1:15,000 - 1:30,000
Immunohistochemistry: 1:500 - 1:2,000
Western Blot: 1:500 - 1:2,000
Other Dilution: User Optimized
Western Blot of Goat anti-RABBIT IgG (H&L) Antibody Pre-adsorbed.Lane 1: Rabbit IgG.Lane 2: None.Load: 100 ng per lane.Primary antibody: RABBIT IgG (H&L) Antibody Pre-adsorbed at 1:1,000 for overnight at 4°C.Secondary antibody: DyLight™ 800 goat secondary antibody 1:20,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa for Rabbit IgG.Other band(s): none.
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