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Western Blot of Goat anti-RABBIT IgG (H&L) Antibody Pre-adsorbed.Lane 1: Rabbit IgG.Lane 2: None.Load: 100 ng per lane.Primary antibody: RABBIT IgG (H&L) Antibody Pre-adsorbed at 1:1,000 for overnight at 4°C.Secondary antibody: DyLight™ 800 goat secondary antibody 1:20,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa for Rabbit IgG.Other band(s): none.
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