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Catalog Products » Other Products » Goat Anti-Rabbit IgG (H&L) [HRP],pAb

Goat Anti-Rabbit IgG (H&L) [HRP],pAb

Description Anti Rabbit HRP Conjugated Secondary Antibodies are ideal for Western Blotting, Immunohistochemistry, ELISA as well as other anibody detection methods.Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins
Host Species Goat
Antigen Species Rabbit
Conjugation Peroxidase (Horseradish)
Immunogen Rabbit IgG whole molecule

Subclass IgG
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity Conjugated Second Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Storage Store secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

ELISA: 1:20,000 - 1:100,000
Immunohistochemistry: 1:1,000 - 1:5,000
Western Blot: 1:2,000 - 1:10,000
Other Dilution: User Optimized

Goat Anti-Rabbit IgG (H&L) [HRP],pAb
Goat Anti-Rabbit IgG (H&L) [HRP],pAb

Rockland secondary antibodies detect rabbit primary antibodies in a variety of platforms. Shown here is a serial 1:1 dilution of control rabbit IgG protein (011-0102, 250ng starting total load) co incubated with Rockland HRP conjugated Goat anti Rabbit IgG (611-103-122) and Dylight 649 conjugated goat anti Rabbit (611-143-122) 1:20K in Blocking Buffer for Fluorescent Western Blotting. Blot was dried and imaged (A) on Biorad Versa Doc (30 sec, DyLight649),(B) LiCor Odyssey Reader (700 nm),(C ) Rewetted incubated with Femtomax 110 reimaged using BioVersaDoc (for 60 sec),(D) Incubated with TMB substrate TMBM for 5 minutes and scanned, and (E) Rewetted for Chemiluminescence and imaged for 90 sec on the BioRad VersaDoc Imager,




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Cat. No. A10160
Size
Price
1 mg $204.00
24 hour
 
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