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Rockland secondary antibodies detect rabbit primary antibodies in a variety of platforms. Shown here is a serial 1:1 dilution of control rabbit IgG protein (011-0102, 250ng starting total load) co incubated with Rockland HRP conjugated Goat anti Rabbit IgG (611-103-122) and Dylight 649 conjugated goat anti Rabbit (611-143-122) 1:20K in Blocking Buffer for Fluorescent Western Blotting. Blot was dried and imaged (A) on Biorad Versa Doc (30 sec, DyLight649),(B) LiCor Odyssey Reader (700 nm),(C ) Rewetted incubated with Femtomax 110 reimaged using BioVersaDoc (for 60 sec),(D) Incubated with TMB substrate TMBM for 5 minutes and scanned, and (E) Rewetted for Chemiluminescence and imaged for 90 sec on the BioRad VersaDoc Imager,
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